The spider fauna of the Ogasawara Islands, oceanic islands located 1,000 km south of mainland Japan was comprehensively examined by the National Museum of Nature and Science in 2011, which revealed ...that approximately 70% of the spider fauna was composed of non-native species. Following the preceding study, however, only descriptions of several new species have been added and no major updates have been made for the overall spider fauna of the Islands.The faunistic survey of spiders conducted on Chichi-jima Island, the largest island in the Ogasawara Islands in 2023 revealed the presence of two ground-dwelling spider species, Oedignatha scrobiculata Thorell, 1881 (Araneae, Liocranidae) and Boagrius qiong Lin & Li, 2022 (Araneae, Palpimanidae) on the Island. This represents the first record of the two species from Japan, the first record of palpimaid spiders from Japan and the initial documentation of liocranid spiders in the Ogasawara Islands.
Volume 25, no. 2, Page 95-102, 2010 Page 98, column 2, line 28 from the top, 'The N-terminal amino acid sequence of the protein was determined as IPDELYEALGVDKYKASPKE. The sequence was identical to ...the deduced sequence of the 31st to 50th residues of the HAO precursor encoded in the noc_0892 gene of the N. oceani ATCC19707' should read 'The N-terminal amino acid sequence of the protein was determined as DIPDELYEALGVDKYKASPK. The sequence was identical to the deduced sequence of the 30th to 49th residues of the HAO precursor encoded in the noc_0892 gene of the N. oceani ATCC19707'.
A Gram-stain-positive and motile bacterial strain, designated IB182363
T
, was isolated from surface seawater of the South China Sea. Cells grew at pH 5.0–9.5 (optimum, pH 7.0–8.0), 20–40 °C ...(optimum, 30 °C) and with 1–8 % (w/v) NaCl (optimum, 2–4 %). On the basis of 16S rRNA gene sequence analysis, strain IB182363
T
was affiliated to the genus
Paenibacillus
and the closest phylogenetically related species was
Paenibacillus ginsengarvi
DSM18677
T
with 96.9 % sequence similarity. The values of whole genome average nucleotide identity analysis and digital DNA–DNA hybridization between the isolate and the closely related type strains were less than 86.3 and 25.6 %, respectively. Chemotaxonomic analysis revealed that strain IB182363
T
possessed
meso
-diaminopimelic acid in the cell-wall peptidoglycan and contained menaquinone MK-7 as the predominant isoprenoid quinone. The major cellular fatty acids were anteiso-C
15 : 0
, C
16 : 0
and iso-C
16 : 0
. The polar lipids comprised phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified glycolipid, two unidentified aminolipids, two unidentified phospholipids and four unidentified aminophospholipids. The genomic DNA G+C content was 54.5 mol%. On the basis of the above results, strain IB182363
T
represents a novel species of the genus
Paenibacillus
, for which we propose the name
Paenibacillus oceani
sp. nov. with the type strain IB182363
T
(=MCCC 1K04630
T
=JCM 34214
T
).
A novel Gram-stain-negative, short rod-shaped, facultatively anaerobic, non-motile, non-gliding, oxidase-positive and catalase-negative bacterium, designated ML27
T
, was isolated from oyster ...homogenate in Rushan, Weihai, PR China. Growth occurred at 20–33 °C (optimum, 30 °C), at pH 7.0–9.0 (optimum, pH 7.5–8.0) and in the presence of 1–6 % (w/v) NaCl (optimum, 3 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain ML27
T
was 90.7 % similar to
Suttonella ornithocola
DSM 18249
T
, 89.2 % to
Suttonella indologenes
JCM 1478
T
and 88.2 % to
Cardiobacterium hominis
DSM 8339
T
; similarities to other species were less than 90 %. The average amino acid identity between strain ML27
T
,
S. indologenes
JCM 1478
T
,
S. ornithocola
DSM 18249
T
,
C. hominis
DSM 8339
T
and
Dichelobacter nodosus
ATCC 25549
T
were 46.23, 45.86, 45.54 and 45.84 %, respectively. Phylogenomic tree and phylogenetic analyses based on 16S rRNA gene sequences showed that the isolate formed a novel family-level clade in the order
Cardiobacteriales
. The sole respiratory quinone was ubiquinone-7 (Q-7). The dominant cellular fatty acids were summed feature 8 (C
18 : 1
ω7
c
/C
18 : 1
ω6
c
; 46.3 %), C
16 : 0
(17.8 %) and summed feature 3 (C
16 : 1
ω7
c
/C
16 : 1
ω6
c
; 13.5 %). The DNA G+C content of strain ML27
T
was 45.6 mol%. Polar lipids included phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and one unidentified lipid. Comparative analyses of 16S rRNA gene sequences, genomic distinctiveness and characterization indicated that strain ML27
T
represents a novel species of a new genus within a novel family of the order
Cardiobacteriales
, for which the name
Ostreibacterium oceani
gen. nov., sp. nov. is proposed. The type strain of
Ostreibacterium oceani
is ML27
T
(=MCCC 1H00372
T
=KCTC 72155
T
). In addition, a novel family,
Ostreibacteriaceae
fam. nov., is proposed to accommodate the genus
Ostreibacterium
.
Strains J15B81-2
T
and J15B91
T
were isolated from a sediment sample collected from the South China Sea. Cells of both strains were observed to be rod-shaped, non-gliding, Gram-stain-negative, ...yellow-pigmented, facultatively anaerobic, catalase-positive, oxidase-negative and showing optimum growth at 30 °C. Strains J15B81-2
T
and J15B91
T
could tolerate up to 9 and 10 % (w/v) NaCl concentration and grow at pH 6.5–9.5 and 6.0–9.0, respectively. The strains shared 97.4 % 16S rRNA gene sequence similarity to each other but were identified as two distinct species based on 81.1–85.8 % ANIb and 31.5 % dDDH values calculated using whole genome sequences. Strains J15B81-2
T
and J15B91
T
shared highest 16S rRNA gene sequence similarity to
Salinimicrobium xinjiangense
CGMCC 1.12522
T
(98.4 %) and
Salinimicrobium sediminis
CGMCC 1.12641
T
(98.0 %), respectively. Among species with validly published names,
S. sediminis
CGMCC 1.12641
T
shared close genetic relatedness with strains J15B81-2
T
85.1–85.3% average nucleotide identity based on
blastBlast
+ (ANIb) and 30.6 % digital DNA–DNA hybridization (dDDH) and J15B91
T
(76.6–79.1 % ANIb and 21.5 % dDDH). The major fatty acid of strains J15B81-2
T
and J15B91
T
were identified as iso-C
15 : 0
and iso-C
16 : 0
, respectively, and the major polar lipids of the two strains consisted of phosphatidylethanolamine, one unidentified phospholipid, one unidentified aminolipid and one unidentified lipid. The strains contained MK-6 as their predominant menaquinone. The genomic G+C contents of strains J15B81-2
T
and J15B91
T
were determined to be 41.7 and 41.8 mol %, respectively. Both strains are considered to represent two novel species of the genus
Salinimicrobium
and the names
Salinimicrobium nanhaiense
sp. nov. and
Salinimicrobium oceani
sp. nov. are proposed for strains J15B81-2
T
(=KCTC 72867
T
=MCCC 1H00410
T
) and J15B91
T
(=KCTC 72869
T
=MCCC 1H00411
T
), respectively.
A novel Gram-stain-negative, aerobic, non-motile, rod-shaped and orange-colored bacterium, designated as strain C305
T
, was isolated from marine sediment of the coast area of Weihai, China. Strain ...C305
T
growth occurs at 4–40 °C (optimally at 30–33 °C), pH 6.0–9.0 (optimally at pH 8.0) and with 0.5–10.0% (w/v) NaCl (optimum 1.5–3.0%). No growth is observed without NaCl. The major cellular fatty acids of strain C305
T
were identified as iso-C
15:0
, iso-C
15:1
G and iso-C
17:0
3-OH. The major respiratory quinone was found to be MK-6, and the DNA G + C content was determined to be 35.5 mol%. The predominant polar lipids were mainly phosphatidylethanolamines (PE), unidentified aminophospholipids (APL), andunidentified lipid (L2). Phylogenetic analysis based on 16S rRNA gene sequences revealed that C305
T
was a member of the genus
Brumimicrobium
and had a 16S rRNA gene sequence similarity values of 96.9–98.0% with recognized
Brumimicrobium
species. On the basis of the phylogenetic and phenotypic evidences, strain C305
T
represents a novel species of the genus
Brumimicrobium
, for which the name
Brumimicrobium oceani
sp. nov. is proposed. The type strain is C305
T
(= KCTC 62371
T
= MCCC 1H00297
T
).
Two novel marine actinobacteria, designated as SCSIO 60955T and SCSIO 61214T, were isolated from deep-sea sediment samples collected from the South China Sea. The cells of these organisms stained ...Gram-negative and were rod shaped. These strains were aerobic, and catalase- and oxidase-positive. Optimal growth occurred at 28 °C and pH 7 over 14 days of cultivation. Both strains possessed phospholipids and phosphoglycolipids. The main menaquinone was MK-7. The major fatty acid was C16:0. The peptidoglycan structure was type A1γ′ (meso-Dpm). Analysis of genome sequences revealed that the genome size of SCSIO 60955T was 3.37 Mbp with G + C content of 76.1%, while the genome size of SCSIO 61214T was 3.67 Mbp with a G + C content of 74.8%. The ANI and 16S rRNA gene analysis results showed that the pairwise similarities between the two strains were 73.4% and 97.7% and that with other recognized Thermoleophilia species were less than 69.1% and 87.8%, respectively. Phylogenetic analysis of the 16S rRNA gene sequences showed that strains SCSIO 60955T and SCSIO 61214T were separately clustered together and formed a well-separated phylogenetic branch distinct from their most related neighbor Gaiella occulta. Based on the data presented here, these two strains are proposed to represent two novel species of a novel genus, for which the name Miltoncostaea marina gen. nov., sp. nov., with the type strain SCSIO 60955T (=DSM 110281T =CGMCC 1.18757T), and Miltoncostaea oceani sp. nov., with the type strain SCSIO 61214T (=KCTC 49527T =CGMCC 1.18758T) are proposed. We also propose that these organisms represent a novel family named Miltoncostaeaceae fam. nov. of a novel order Miltoncostaeales ord. nov.
A novel rod-shaped, Gram-stain-negative, aerobic bacterium, designated S35
, was isolated from deep-sea sediment collected from the Pacmanus hydrothermal field, Manus Basin, Papua New Guinea. Strain ...S35
grew optimally at 28 °C, at pH 7.0-8.0 and in the presence of 2.0 % (w/v) NaCl. 16S rRNA gene sequence analysis indicated that strain S35
shared 97.38-98.55% similarity with the type strains of Alteromonas lipolytica, Alteromonas mediterranea and Aestuariibacterhalophilus. Phylogenetic analysis showed that strain S35
belonged to the genus Alteromonas. The strain contained ubiquinone-8 as the predominant respiratory lipoquinone. Summed feature 3 (C16 : 1ω7c and/or C16 : 1ω7c), summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and C16 : 0 were the major fatty acids. The DNA G+C content of strain S35
was 51.3 mol%. These results indicated that strain S35
represents a novel species of the genus Alteromonas, for which the name Alteromonas oceani sp. nov. (type strain S35
=KCTC 52449
=CGMCC 1.16029
) is proposed.
Display omitted
•A thermostable d-lyxose isomerase was characterized from T. oceani.•It displayed maximal activity in presence of 1mM Mn2+ at pH 6.5 and 65°C.•The half-life was 5.64, 2.82, 0.77, and ...0.2h at 70, 75, 80, and 85°C, respectively.
d-Mannose has prebiotic effect and potential medical application. Besides, it can be used as substrate to produce mannitol, a functional polyol widely used in food industry. As this result, it has attracted many researchers’ attention. In this work, a thermostable d-mannose-producing d-lyxose isomerase (D-LI) was characterized from a hyperthermophile, Thermosediminibacter oceani. The recombinant D-LI could be remarkably activated by Mn2+. It displayed maximal activity in presence of 1mM Mn2+ at pH 6.5 and 65°C, and was determined to be highly thermostable at 80°C. The half-life was calculated to be 5.64, 2.82, 0.77, and 0.2h at 70, 75, 80, and 85°C, respectively. The enzyme showed the optimum activity using d-lyxose as substrate and could also effectively catalyze the isomerization between d-fructose and d-mannose. Under optimum conditions, 101.6g/L d-mannose was produced from 400g/L d-fructose after reaction for 9h, giving a conversion yield of 25.4%.
BACKGROUND AND OBJECTIVES: Mangroves are known to contain tannins, flavonoids, and quinones, which have the potential to be antibacterial, effective even against multidrug-resistant bacteria. ...Mangroves also have antifungal and antiviral properties. Although, mangroves are known for their use as medicinal ingredients, information regarding symbiont bacteria's antibacterial and antifungal potential is still scarce. Therefore, this study aimed to examine symbiont bacteria in the fruit and leaves of Xylocarpus granatum as additional raw materials for anti-acne cosmetic creams and moisturisers. METHODS: Symbiont bacteria were isolated using the pour plate method through Zobell 2216E and incubated for 2 x 24 hours at 27.5 Celcius degree. Afterwards, 13 isolates were successfully isolated and characterised based on their morphology. Further, everal tests were conducted, including the antibacterial test, antifungal test, molecular identification, and gas chromatography-mass spectrometry. The pathogenic bacteria used in the antibacterial test were Staphylococcus aureus, Vibrio harveyi, and Vibrio alginolyticus FINDINGS: The antibacterial test results showed that eight isolates were capable of producing an inhibition zone against S. aureus, seven isolates were positive for antibacterial activity against Vibrio harveyi, and 10 isolates were positive for antibacterial activity against Vibrio alginolyticus. The pathogenic fungi used in the antifungal test were Malassezia furfur and Candida albicans. The antifungal test results demonstrated that six isolates could produce inhibition zones against Malassezia furfur and Candida albicans. Furthermore, molecular identification was carried out on six potential isolates based on the antibacterial and antifungal tests, which were X2.52, X1.65, X1.64, X1.53, X1.54, and X1.63. The molecular identification results revealed the occurrence of four species in the Xylocarpus granatum mangroves, namely, Sinomicrobium oceani, Proteus mirabilis, Pseudomonas khazarica, and Alcaligenes aquatilis. CONCLUSION: The study found that the mangrove symbiont bacteria had antibacterial and antifungal potential. The compound with the highest concentration in six isolates was 9-octadecenoic acid, methyl ester. This type of content has antibacterial potential and is also predicted to have antifungal potential.
PDF
