The current study was conducted to test cytokinin type and concentration, and source of explant on shoot multiplication of pineapple plant (Ananas comosus ‘Queen’) in vitro during 22/6/2009 to ...1/5/2010. The results of the first experiment showed that axillary buds delayed to grow during 4-5 months of culturing in MS medium supplemented with different concentrations of BA (1.0, 1.5, 2.0, 2.5 or 3.0 mg l
). The MS medium supplemented with 1.0 mg l
BA has led to the vegetative growth of axillary buds. The other concentrations of BA added to MS medium led to callus growth. The results showed that MS medium supplemented with 1.0 mg l
BA and 0.2 mg l
NAA gave adventitious shoots directly after two months from culturing of callus. The results of the second experiment showed that nodal segments cultured in MS medium supplemented with 1.0 mg l
kinetin was a significantly superior on other treatments studied (0.5 mg l-1 kinetin, 0.5 and 1.0 mg l
BA) in number of shoots/explant, leaf length, number of leaves/shoot and leaf area of formation shoot which reached 18.60 shoot/explant, 5.38 cm, 10.60 leaves/shoot and 3.64 cm
, respectively. The results showed that kinetin was a significant superior in all vegetative characteristics of shoots compared with BA. Abbreviation: BA: 6-benzyl adenine.
NAA: α-naphthalene acetic acid.
MS: Murashige and Skoog salts (Murashige and Skoog, 1962)
Raziskava je bila izvedena z namenom testiranja različnih vrst in koncentracij citokininov ter vira stebelnih izsečkov na in vitro razmnoževanje ananasa (Ananas comosus ‘Queen’) v obdobju 22/6/2009 do 1/5/2010. Rezultati prvega poskusa so pokazali, da je rast zalistnih brstov zaostajala prvih 4-5 mesecev gojenja na MS gojišču, ki so mu dodali različne koncentracije BA (1,0, 1,5, 2,0, 2,5 ali 3,0 mg l
). MS gojišče, ki so mu dodali 1,0 mg l
BA je vzpodbudilo rast zalistnih brstov. Druge koncentracije BA, dodane MS gojišču so vodile le k rasti kalusa. Nadaljnji rezultati so pokazali, da so se razvili na MS gojišču, ki sta mu bila dodana 1,0 mg l
BA in 0,2 mg l
NAA poganjki neposredno iz kalusa po dveh mesecih gojenja. Rezultati drugega poskusa so pokazali, da so bili nodijski izsečki, gojeni na MS gojišču, ki so mu dodali 1,0 mg l
kinetina značilno boljši kot drugi postopki, izvedeni v tej raziskavi (0,5 mg l
kinetina, 0,5 in 1,0 mg l
BA) v številu poganjkov/izseček, dolžini listov, številu listov/poganjek in listni površini nastalih poganjkov. Nastalo je 18,60 poganjkov/izseček, 10,60 listov/poganjek, dolžine 5,38 cm in 3,64 cm
površine. Rezultati so pokazali, da je dalo obravnavanje s kinetinom boljše rezultate glede vrednosti vseh merjenih vegetativnih lastnosti poganjkov v primerjavi z BA. Okrajšave: BA: 6-benzil adenin.
NAA: α-naftalen ocetna kislina.
MS: Murashige in Skoog bazalno gojišče (Murashige in Skoog, 1962)
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