• Plant tannins, including condensed tannins (CTs) and hydrolyzable tannins (HTs), are widely distributed in the plant kingdom. To date, tannase (TA) – is a type of tannin acyl-hydrolase hydrolyzing ...HTs, CT monomer gallates and depsides – has been reported in microbes only. Whether plants express TA remains unknown.
• Herein, we report plant TA genes. A native Camellia sinensis TA (CsTA) is identified from leaves. Six TAs are cloned from tea, strawberry (Fragaria × ananassa, Fa) and four other crops. Biochemical analysis shows that the native CsTA and six recombinant TAs hydrolyze tannin compounds, depsides and phenolic glycosides.
• Transcriptional and metabolic analyses reveal that the expression of CsTA is oppositely associated with the accumulation of galloylated catechins. Moreover, the transient overexpression and RNA interference of FaTA are positively associated with the accumulation of ellagitannins in strawberry fruit. Phylogenetic analysis across different kingdoms shows that 29 plant TA homologs are clustered as a plant-specific TA clade in class I carboxylesterases. Further analysis across the angiosperms reveals that these TA genes are dispersed in tanninrich plants, which share a single phylogenetic origin c. 120 million yr ago.
• Plant TA is discovered for the first time in the plant kingdom and is shown to be valuable to improve tannin compositions in plants.
From cyanobacteria to Archaeplastida Selim, Khaled A.; Ermilova, Elena; Forchhammer, Karl
The New phytologist,
08/2020, Letnik:
227, Številka:
3
Journal Article
Recenzirano
Odprti dostop
The PII superfamily consists of signal transduction proteins found in all domains of life. Canonical PII proteins sense the cellular energy state through the competitive binding of ATP and ADP, and ...carbon/nitrogen balance through 2-oxoglutarate binding. The ancestor of Archaeplastida inherited its PII signal transduction protein from an ancestral cyanobacterial endosymbiont. Over the course of evolution, plant PII proteins acquired a glutamine-sensing C-terminal extension, subsequently present in all Chloroplastida PII proteins. The PII proteins of various algal strains (red, green and nonphotosynthetic algae) have been systematically investigated with respect to their sensory and regulatory properties. Comparisons of the PII proteins from different phyla of oxygenic phototrophs (cyanobacteria, red algae, Chlorophyta and higher plants) have yielded insights into their evolutionary conservation vs adaptive properties. The highly conserved role of the controlling enzyme of arginine biosynthesis, N-acetyl-L-glutamate kinase (NAGK), as a main PII-interactor has been demonstrated across oxygenic phototrophs of cyanobacteria and Archaeplastida. In addition, the PII signalling system of red algae has been identified as an evolutionary intermediate between that of Cyanobacteria and Chloroplastida. In this review, we consider recent advances in understanding metabolic signalling by PII proteins of the plant kingdom.
The myo-inositol biosynthesis pathway triggering protein MIPS is best known for its necessity, ubiquitous nature and occurrence throughout all living kingdom. However, the functional disparity of ...MIPS genes in green plant is still viable. The present work considered a comprehensive genome-wide analysis from sequenced plants to identify MIPS homologs in respective organisms and their genomic architecture. Variation of MIPS gene expression in twelve different species in diverse conditions has also been analysed. All MIPS genes share a conserved sequence property in most of its coding region, but its regulatory elements, gene structure and expression network vary significantly. Phylogenetic inference confirms the evolution of MIPS from a single common algal ancestor to seed plants and acquiring functional variation through genomic control. This paper represents MIPS as a model for studying gene duplication, functional divergence and diversification events in plant lineages.
•Variable number of MIPS copies exist in different plant groups evolved through genome duplication and diversification.•Expression of MIPS paralogs in a species varies spatially and temporally.•Plant MIPS genes are mostly conserved, but its regulatory elements, gene structure and expression network vary significantly.•Evolution of MIPS occurred from an algal ancestor to seed plants acquiring functional variation through genomic control.
This open-access edited book is a collection of 17 chapters, synthesized primarily from the lectures delivered by eminent Indian and international experts during a series of capacity-building ...programmes organised in India during 2020 and 2021 under the aegis of 'Indo-German Cooperation on Seed Sector Development', a component of the Bilateral Cooperation between the Governments of India and Germany. Seed Science and Technology, a multi-disciplinary subject, is advancing rapidly keeping pace with the development of improved plant varieties and other climate-resilient technologies. Knowledge of the underlying biological processes and application of appropriate technologies for variety maintenance and seed production; quality assurance, testing and enhancement; processing, packaging and storage etc., are important in a seed programme. Chapters presented in the book is a blend of basic seed biology covering seed development, maturation, dormancy, germination, vigour and invigoration, and seed deterioration; variety maintenance and production of genetically pure seed of open-pollinated and hybrid varieties in a few key field crops and vegetables, and fundamentals of seed processing, packaging and storage; and seed quality assurance systems followed in different countries; testing the essential components of seed quality including seed health, application of molecular technologies for precision in testing, and enhancement of seed quality. It concludes by identifying the key areas of future seed research and technology development. The book covers the fundamentals and recent advances of seed science and technology with the latest research information and an exhaustive and updated list of references on different topics. It is expected to benefit the students as well as the scientists, faculty members and seed sector professionals, working in the public and private seed sectors, certification authorities and seed producing agencies in India, and elsewhere.
We have recently described 'Cytobacts' as abundant intracellular endophytic bacteria inhabiting live plant cells based on the observations with callus and cell suspension cultures of grapevine and ...other plant species with the origin ascribable to field explants. In this study, we investigated the prevalence of such cytoplasmic bacterial associations in field plants across different taxa, their cultivability, and the extent of taxonomic diversity and explored the possibility of their embryo-mediated vertical transmission. Over 100 genera of field plants were surveyed for 'Cytobacts' through bright-field live-cell imaging as per our previous experience using fresh tissue sections from surface-sterilized shoot-tissues with parallel cultivation-based assessments. This revealed widespread cellular bacterial associations visualized as copious motile micro-particles in the cytoplasm with no or sparse colony forming units (CFU) from the tissue-homogenates indicating their general non-cultivability. Based on the ease of detection and the abundance of 'Cytobacts' in fresh tissue sections, the surveyed plants were empirically classified into three groups: (i) motile bacteria detected instantly in most cells; (ii) motility not so widely observed, but seen in some cells; and (iii) only occasional motile units observed, but abundant non-motile bacterial cells present. Microscopy versus 16S-rRNA V3-V4 amplicon profiling on shoot-tip tissues of four representative plants-tomato, watermelon, periwinkle, and maize-showed high bacterial abundance and taxonomic diversity (11-15 phyla) with the dominance of Proteobacteria followed by Firmicutes/Actinobacteria, and several other phyla in minor shares. The low CFU/absence of bacterial CFU from the tissue homogenates on standard bacteriological media endorsed their cultivation-recalcitrance. Intracellular bacterial colonization implied that the associated organisms are able to transmit vertically to the next generation through the seed-embryos. Microscopy and 16S-rRNA V3-V4 amplicon/metagenome profiling of mature embryos excised from fresh watermelon seeds revealed heavy embryo colonization by diverse bacteria with sparse or no CFU. Observations with grapevine fresh fruit-derived seeds and seed-embryos endorsed the vertical transmission by diverse cultivation-recalcitrant endophytic bacteria (CREB). By and large, Proteobacteria formed the major phylum in fresh seed-embryos with varying shares of diverse phyla. Thus, we document 'Cytobacts' comprising diverse and vertically transmissible CREBs as a ubiquitous phenomenon in vascular plants.
The phylogenetic positions of the primary photosynthetic eukaryotes, or Archaeplastida (green plants, red algae, and glaucophytes) and the secondary photosynthetic chromalveolates, Haptophyta, vary ...depending on the data matrices used in the previous nuclear multigene phylogenetic studies. Here, we deduced the phylogeny of three groups of Archaeplastida and Haptophyta on the basis of sequences of the multiple slowly evolving nuclear genes and reduced the gaps or missing data, especially in glaucophyte operational taxonomic units (OTUs). The present multigene phylogenetic analyses resolved that Haptophyta and two other groups of Chromalveolata, stramenopiles and Alveolata, form a monophyletic group that is sister to the green plants and that the glaucophytes and red algae are basal to the clade composed of green plants and Chromalveolata. The bootstrap values supporting these phylogenetic relationships increased with the exclusion of long-branched OTUs. The close relationship between green plants and Chromalveolata is further supported by the common replacement in two plastid-targeted genes.
The induction of resistance to disease during plant development is widespread in the plant kingdom. Resistance appears at different stages of host development, varies with plant age or tissue ...maturity, may be specific or broad-spectrum and is driven by diverse mechanisms, depending on plant-pathogen interactions. Studies of these forms of resistance may help us to evaluate more exhaustively the plethora of levels of regulation during development, the variability of the defense potential of developing hosts and may have practical applications, making it possible to reduce pesticide applications. Here, we review the various types of developmental resistance in plants and current knowledge of the molecular and cellular processes involved in their expression. We discuss the implications of these studies, which provide new knowledge from the molecular to the agrosystem level. Summary 405 I. Introduction 405 II. The many forms of developmental resistance 406 III. Molecular mechanisms of developmental resistance 410 IV. Relationships between defense and development in plants 412 V. Concluding remarks 413 Acknowledgements 413 References 413
There has been a recent surge of interest in remote sensing and its use in ecology and conservation. This book focuses explicitly on the Normalized Difference Vegetation Index (NDVI), a simple ...numerical indicator and powerful tool that can be used to assess spatio-temporal changes in green vegetation. The NDVI opens the possibility of addressing questions on scales inaccessible to ground-based methods alone; it is mostly freely available with global coverage over several decades. This text provides an authoritative overview of the principles and possible applications of the NDVI in ecology, environmental and wildlife management, and conservation. NDVI data can provide valuable information about temporal and spatial changes in vegetation distribution, productivity, and dynamics; allowing monitoring of habitat degradation and fragmentation, or assessment of the ecological effects of climatic disasters such as drought or fire. The NDVI has also provided ecologists with a promising way to couple vegetation with animal distribution, abundance, movement, survival and reproductive parameters. Over the last few decades, numerous studies have highlighted the potential key role of satellite data and the NDVI in macroecology, plant ecology, animal population dynamics, environmental monitoring, habitat selection and habitat use studies, and paleoecology. The chapters are organized around two sections: the first detailing vegetation indices and the NDVI, the principles behind the NDVI, its correlation with climate, the available NDVI datasets, and the possible complications and errors associated with the use of this satellite-based vegetation index. The second section discusses the possible applications of the NDVI in ecology, environmental and wildlife management, and conservation.
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