Epigallocatechin gallate (EGCG) is associated with various health benefits. In this review, we searched current work about the effects of EGCG and its wound dressings on skin for wound healing. ...Hydrogels, nanoparticles, micro/nanofiber networks and microneedles are the major types of EGCG-containing wound dressings. The beneficial effects of EGCG and its wound dressings at different stages of skin wound healing (hemostasis, inflammation, proliferation and tissue remodeling) were summarized based on the underlying mechanisms of antioxidant, anti-inflammatory, antimicrobial, angiogenesis and antifibrotic properties. This review expatiates on the rationale of using EGCG to promote skin wound healing and prevent scar formation, which provides a future clinical application direction of EGCG.
Hydrogels with high mechanical strength and injectability have attracted extensive attention in biomedical and tissue engineering. However, endowing a hydrogel with both properties is challenging ...because they are generally inversely related. In this work, by constructing a multi‐hydrogen‐bonding system, a high‐strength and injectable supramolecular hydrogel is successfully fabricated. It is constructed by the self‐assembly of a monomeric nucleoside molecular gelator (2‐amino‐2′‐fluoro‐2′‐deoxyadenosine (2‐FA)) with distilled water/phosphate buffered saline as solvent. Its storage modulus reaches 1 MPa at a concentration of 5.0 wt%, which is the strongest supramolecular hydrogel comprising an ultralow‐molecular‐weight (MW < 300) gelator. Furthermore, it exhibits excellent shear‐thinning injectability, and completes the sol–gel transition in seconds after injection at 37 °C. The multi‐hydrogen‐bonding system is essentially based on the synergistic interactions between the double NH2 groups, water molecules, and 2′‐F atoms. Furthermore, the 2‐FA hydrogel exhibits excellent biocompatibility and antibacterial activity. When applied to rat molar extraction sockets, compared to natural healing and the commercial hemorrhage agent gelatin sponge, the 2‐FA hydrogel exhibits faster degradation and induces less osteoclastic activity and inflammatory infiltration, resulting in more complete bone healing. In summary, this study provides ideas for proposing a multifunctional, high‐strength, and injectable supramolecular hydrogel for various biomedical engineering applications.
Simultaneously achieving high strength and injectability in one hydrogel is challenging. A high strength and injectable supramolecular hydrogel is constructed by the self‐assembly of a monomeric nucleoside molecular gelator (2‐amino‐2′‐fluoro‐2′‐deoxyadenosine (2‐FA)). Notably, it exhibits excellent biocompatibility, fast biodegradation, antibacterial property, and osteoclast inhibition, making it a potential candidate biomaterial for tooth‐extraction wound healing and tissue engineering.
Treatment Strategies for Infected Wounds Negut, Irina; Grumezescu, Valentina; Grumezescu, Alexandru Mihai
Molecules (Basel, Switzerland),
09/2018, Letnik:
23, Številka:
9
Journal Article
Recenzirano
Odprti dostop
The treatment of skin wounds is a key research domain owing to the important functional and aesthetic role of this tissue. When the skin is impaired, bacteria can soon infiltrate into underlying ...tissues which can lead to life-threatening infections. Consequently, effective treatments are necessary to deal with such pathological conditions. Recently, wound dressings loaded with antimicrobial agents have emerged as viable options to reduce wound bacterial colonization and infection, in order to improve the healing process. In this paper, we present an overview of the most prominent antibiotic-embedded wound dressings, as well as the limitations of their use. A promising, but still an underrated group of potential antibacterial agents that can be integrated into wound dressings are natural products, especially essential oils. Some of the most commonly used essential oils against multidrug-resistant microorganisms, such as tea tree, St. John's Wort, lavender and oregano, together with their incorporation into wound dressings are presented. In addition, another natural product that exhibits encouraging antibacterial activity is honey. We highlight recent results of several studies carried out by researchers from different regions of the world on wound dressings impregnated with honey, with a special emphasis on Manuka honey. Finally, we highlight recent advances in using nanoparticles as platforms to increase the effect of pharmaceutical formulations aimed at wound healing. Silver, gold, and zinc nanoparticles alone or functionalized with diverse antimicrobial compounds have been integrated into wound dressings and demonstrated therapeutic effects on wounds.
•The fabrication of electrospun chitosan nanofibers with random and aligned fibrous structure were introduced.•The as-crosslinked electrospun chitosan nanofibers possess enhanced wet stability, ...improved mechanical property and good biocompatibility.•The recent studies on the wound healing application electrospun chitosan fibers were reviewed in the work.
Electrospun nanofibers have been considered as relatively ideal material system for the design and construction of surgical wound dressings in the past few decades. Although there are lots of other biopolymers employed for electrospinning, chitosan is one of the most important biopolymers for the fabrication of electrospun nanofibers, due to its nontoxic, antibacterial, biocompatible as well as biodegradable properties. Electrospun chitosan nanofibers with random or aligned fiber structure can further be cross-linked by physical, chemical and ionic crosslinking methods. The as-crosslinked electrospun chitosan nanofibrous membranes possess enhanced wet stability, improved mechanical property and good biocompatibility, which are necessary for wound healing application. The present work reviews the recent studies on the design and development of electrospun chitosan nanofibers and their applications for wound healing, such as antibacterial materials, hemostatic dressings, angiogenesis and immune regulation.
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Fibroblasts are highly dynamic cells that play a central role in tissue repair and fibrosis. However, the mechanisms by which they contribute to both physiologic and pathologic states of ...extracellular matrix deposition and remodeling are just starting to be understood. In this review article, we discuss the current state of knowledge in fibroblast biology and heterogeneity, with a primary focus on the role of fibroblasts in skin wound repair. We also consider emerging techniques in the field, which enable an increasingly nuanced and contextualized understanding of these complex systems, and evaluate limitations of existing methodologies and knowledge. Collectively, this review spotlights a diverse body of research examining an often-overlooked cell type—the fibroblast—and its critical functions in wound repair and beyond.
Talbott et al. discuss the current state of knowledge in fibroblast biology and heterogeneity, primarily focusing on fibroblasts in skin wound repair. They also consider emerging techniques in the field and evaluate limitations of existing methodologies and knowledge.
Immunology of Acute and Chronic Wound Healing Raziyeva, Kamila; Kim, Yevgeniy; Zharkinbekov, Zharylkasyn ...
Biomolecules (Basel, Switzerland),
05/2021, Letnik:
11, Številka:
5
Journal Article
Recenzirano
Odprti dostop
Skin wounds greatly affect the global healthcare system, creating a substantial burden on the economy and society. Moreover, the situation is exacerbated by low healing rates, which in fact are ...overestimated in reports. Cutaneous wounds are generally classified into acute and chronic. The immune response plays an important role during acute wound healing. The activation of immune cells and factors initiate the inflammatory process, facilitate wound cleansing and promote subsequent tissue healing. However, dysregulation of the immune system during the wound healing process leads to persistent inflammation and delayed healing, which ultimately result in chronic wounds. The microenvironment of a chronic wound is characterized by high quantities of pro-inflammatory macrophages, overexpression of inflammatory mediators such as TNF-α and IL-1β, increased activity of matrix metalloproteinases and abundance of reactive oxygen species. Moreover, chronic wounds are frequently complicated by bacterial biofilms, which perpetuate the inflammatory phase. Continuous inflammation and microbial biofilms make it very difficult for the chronic wounds to heal. In this review, we discuss the role of innate and adaptive immunity in the pathogenesis of acute and chronic wounds. Furthermore, we review the latest immunomodulatory therapeutic strategies, including modifying macrophage phenotype, regulating miRNA expression and targeting pro- and anti-inflammatory factors to improve wound healing.
Chronic refractory wounds are a multifactorial comorbidity of diabetes mellitus with the characteristic of impaired vascular networks. Currently, there is a lack of effective treatments for such ...wounds. Various types of mesenchymal stem cell-derived exosomes (MSC-exos) have been shown to exert multiple therapeutic effects on skin regeneration. We aimed to determine whether a constructed combination of human umbilical cord MSC (hUCMSC)-derived exosomes (hUCMSC-exos) and Pluronic F-127 (PF-127) hydrogel could improve wound healing.
We topically applied human umbilical cord-derived MSC (hUCMSC)-derived exosomes (hUCMSC-exos) encapsulated in a thermosensitive PF-127 hydrogel to a full-thickness cutaneous wound in a streptozotocin-induced diabetic rat model. The material properties and wound healing ability of the hydrogel and cellular responses were analyzed.
Compared with hUCMSC-exos, PF-127-only or control treatment, the combination of PF-127 and hUCMSC-exos resulted in a significantly accelerated wound closure rate, increased expression of CD31 and Ki67, enhanced regeneration of granulation tissue and upregulated expression of vascular endothelial growth factor (VEGF) and factor transforming growth factor beta-1 (TGFβ-1).
The efficient delivery of hUCMSC-exos in PF-127 gel and improved exosome ability could promote diabetic wound healing. Thus, this biomaterial-based exosome therapy may represent a new therapeutic approach for cutaneous regeneration of chronic wounds.
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