Imunokemično odkrivanje antigena PCN in Ki-67 na celičnih vzorcih raka dojke : magistrska naloga
Srebotnik-Kirbiš, Irena
Background: Assessment of breast cancer proliferative activity before surgery could help in planning effective individual treatment protocols, particulary in planning neo-adjuvant chemoterapy. In the ...present study, we are aiming to find out whether it is possible to immunochemically assess the proliferative activity of tumor on fine needle aspirates before operation. The preparation and fixation of the cell samples of immunochemical methods have not been standarized and, in addition to that, they differ from the preparation of tissue samplesection of two most important proliferative antigens, PCNA and Ki-67, in breast cancer cell samples. Out particular attention has been paid to detection of these antigens in diagnostic Papanicolaou stained fine needle aspirates. Materials and Methods: The cytospared. Part of them were fixed in Delaunayćs fixative or its modification and stained according to Papanicolaou.Others were fixed by Mercofix, methanol, acetone or glutaraldehyde and kept in methanol. Immunochemical detection of PCNA and Ki-67 antigens was carried out by avidin-biotin peroxidase method with PC10 and MIB-1 monoclonal antibodies, respectively. The percentage oIB-1 positive cells was evaluated semiquantitatively and was compared to the S phase fraction calculated from MCF-7 flow cytometric DNA histogram. Results; On the samples stained by Papanicolaoućs procedure antigen Ki-67 could be immunochemically detected onrowaves was not required for samples fixed in methanol. All other fixation methods had a negative effect on the Ki-67 antigen detection.(Abstract truncated at 2000 characteres.)
Vrsta gradiva - magistrsko delo
Založništvo in izdelava - Ljubljana : [I. Srebotnik Kirbiš], 1998
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