(UL)
-
Vpliv encimskega ekstrakta riževe semenske ovojnice na rast, razvoj in preživetje mezenhimskih matičnih celic, izoliranih iz maščobnega tkiva podgane = Effect of enzymatic extract from rice bran on growth, differentiation and survival of rat adipose tissue-derived mesenchymal stem cells : masters's thesis : Master's Study of Laboratory biomedicineŠkof, Maja, biokemičarkaEnzymatic extract of rice bran (EERB) is water-soluble extract that exerts strong antioxidant activity. It is obtained after enzyme hydrolysis of rice bran with endoproteases. It contains a mixture ... of water-soluble substances, rich in antioxidants (γ-oryzanol, vitamin E) and consists mainly of peptides, polysaccharides and polyunsaturated fatty acids. Adipose tissue-derived mesenchymal stem cells (ADSC) are multipotent stem cells, which are a prominent source in regenerative medicine. Because of their relatively easy isolation and broad differentiation potential, many studies are focusing on different tissue regeneration with ADSC. However, many difficulties in their growth and differentiation still exist. This Master’s thesis is a preliminary study where we evaluated the effect of EERB on different characteristics of ADSC. We researched how EERB as a cell culture medium influences proliferation of ADSC, their differentiation to neurospheres and neurons and if EERB as an antioxidant protects ADSC against oxidative stress, induces by cumene hydroperoxide (CH). EERB as a cell culture medium supplement has shown to be beneficial for growth of ADSC. In adequate concentrations EERB promotes cell growth, as we have shown with MTS assay and cell counting experiments. Also, EERB does influence ADSC gene expression, as we researched for SOX2 and eEF. In regenerative medicine, ADSC are of great interest for differentiation to neurons, though the process is arduous and strongly depended on environment and cell conditions. EERB could be an important factor in more efficient differentiation because of its antioxidative features though more research should be done on this aspect. Additionally, we showed that EERB protects ADSC against oxidative stress, that was induced by CH. However, more research on this topic is needed. The results of MTS assay and cell counting showed that EERB does protect against oxidative stress, but LDH cytotoxicity assay was showing stronger cytotoxicity when cells were incubated with CH and EERB. We can conclude that EERB is indeed a promising new factor for cells proliferation and its influence should be considered in future research.Vrsta gradiva - magistrsko delo ; neleposlovje za odrasleZaložništvo in izdelava - Ljubljana : [M. Škof], 2024Jezik - angleškiCOBISS.SI-ID - 187568387
Avtor
Škof, Maja, biokemičarka
Drugi avtorji
Marc, Janja |
Cano Rodríguez, Maria Mercedes
Teme
Mezenhimske matične celice |
Oksidativni stres |
encimski ekstrakt riževe ovojnice |
proliferacija
![loading ... loading ...](themes/default/img/ajax-loading.gif)
Knjižnica | Signatura – lokacija, inventarna št. ... | Status izvoda |
---|---|---|
Fakulteta za farmacijo, Ljubljana | Knjižnica mlab 60 ŠKOF MAJA Vpliv 361 IN: 0018084 |
prosto - za čitalnico |
![loading ... loading ...](themes/default/img/ajax-loading.gif)
![loading ... loading ...](themes/default/img/ajax-loading.gif)
![loading ... loading ...](themes/default/img/ajax-loading.gif)
Vnos na polico
Trajna povezava
- URL:
Faktor vpliva
Dostop do baze podatkov JCR je dovoljen samo uporabnikom iz Slovenije. Vaš trenutni IP-naslov ni na seznamu dovoljenih za dostop, zato je potrebna avtentikacija z ustreznim računom AAI.
Leto | Faktor vpliva | Izdaja | Kategorija | Razvrstitev | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Baze podatkov, v katerih je revija indeksirana
Ime baze podatkov | Področje | Leto |
---|
Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
---|---|
Škof, Maja, biokemičarka | ![]() |
Marc, Janja | 12189 |
Cano Rodríguez, Maria Mercedes | ![]() |
Vir: Osebne bibliografije
in: SICRIS
Izberite prevzemno mesto:
Prevzem gradiva po pošti
Naslov za dostavo:
Med podatki člana manjka naslov.
Storitev za pridobivanje naslova trenutno ni dostopna, prosimo, poskusite še enkrat.
S klikom na gumb "V redu" boste potrdili zgoraj izbrano prevzemno mesto in dokončali postopek rezervacije.
S klikom na gumb "V redu" boste potrdili zgoraj izbrano prevzemno mesto in naslov za dostavo ter dokončali postopek rezervacije.
S klikom na gumb "V redu" boste potrdili zgoraj izbrani naslov za dostavo in dokončali postopek rezervacije.
Obvestilo
Trenutno je storitev za avtomatsko prijavo in rezervacijo nedostopna. Gradivo lahko rezervirate sami na portalu Biblos ali ponovno poskusite tukaj kasneje.
Gesla v Splošnem geslovniku COBISS
Izbira mesta prevzema
Gradivo iz matične enote je brezplačno. Če je gradivo na mesto prevzema dostavljeno iz drugih enot, lahko knjižnica to storitev zaračuna.
Mesto prevzema | Status gradiva | Rezervacija |
---|
Rezervacija v teku
Prosimo, počakajte trenutek.
Rezervacija je uspela.
Rezervacija ni uspela.
Rezervacija...
Članska izkaznica:
Mesto prevzema: