(UL)
-
Študij nevrotoksičnega mesta na molekuli fosfolipaz A2 : magistrsko deloIvanovski, GabrielaVipera a. ammodytes venom contains three presynaptically neurotoxic PLA2s, ammodytoxins (atx) A, B and C, among which atxA is the most lethal. Snake venom PLA2s have been effectively produced in the ... Escherichia coli expression system by using a plasmid with strong T7 promotor. Recombinant toxins can be produced in the bacterial cytoplasm either as mature proteins or as fusion proteins. The latter must be activated in vitro by cleaving the N-terminal propeptide. For this purpose trypsin is usually used. Since ammodytoxins and trypsin have similar isoelectric points, isolation of ammodytoxins is difficult. We therefore carried out the activation with acetylated trypsin, which is an affective activating enzyme, easily separated from recombinant atxC by cation exchange chromatography. The enzymatic activity against egg yolk substrate was used to monitor correct folding. The specific enzymatic activity atxC is the same as that of naturally occuring atxC, 286 Užmg, although its LD50 is slightly lower, 0.16 mgžkg of mause. The structure-function relationships of presynaptically neurotoxic PLA2s have not yet been elucidated. We have used site-directed mutagenesis to determine at least part of the molecule that is involved in the process of neurotoxicity. Attention has been focussed on the region 115-119, where atxA and the 28-timesless toxic atxB differ. Using polymerase chain reaction, we prepared four mutants of atxAČ the quadruple mutant atxA (Y115KžI16KžR118MžN119L) (atxAKKML), and three double mutants atxA (Y115KžI116K) (atxA KK), atxA (R118MžM119L) (atxA ML) and atxA (R118MžN119F) (atxA MF). All were expressed as fusion proteins. After isolation and renaturation they were activated with acetylated trypsin and purified by cation exchange chromatography. Abstract truncated at 2000 characters.Vrsta gradiva - magistrsko deloZaložništvo in izdelava - Ljubljana : [G. Ivanovski], 1999Jezik - slovenskiCOBISS.SI-ID - 1996564
Avtor
Ivanovski, Gabriela
Drugi avtorji
Pungerčar, Jože, 1960-
Teme
Viper venoms |
Chemistry |
Phospholipases A |
Chemistry |
Phospholipases A |
Isolation and purification |
Modrasji strup |
Kemija |
Fosfolipaze A |
Kemija |
Fosfolipaze A |
Izolacija in čiščenje |
biokemija |
toksikologija
![loading ... loading ...](themes/default/img/ajax-loading.gif)
Knjižnica | Signatura – lokacija, inventarna št. ... | Status izvoda |
---|---|---|
MF, Centralna medicinska knjižnica, Ljubljana | magistrske naloge IVANOVSKI Gabriela Študij IN: 099900559 |
prosto - na dom, čas izposoje: 1 mes. |
![loading ... loading ...](themes/default/img/ajax-loading.gif)
![loading ... loading ...](themes/default/img/ajax-loading.gif)
![loading ... loading ...](themes/default/img/ajax-loading.gif)
Vnos na polico
Trajna povezava
- URL:
Faktor vpliva
Dostop do baze podatkov JCR je dovoljen samo uporabnikom iz Slovenije. Vaš trenutni IP-naslov ni na seznamu dovoljenih za dostop, zato je potrebna avtentikacija z ustreznim računom AAI.
Leto | Faktor vpliva | Izdaja | Kategorija | Razvrstitev | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Baze podatkov, v katerih je revija indeksirana
Ime baze podatkov | Področje | Leto |
---|
Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
---|---|
Ivanovski, Gabriela | 15968 |
Pungerčar, Jože, 1960- | 04570 |
Vir: Osebne bibliografije
in: SICRIS
Izberite prevzemno mesto:
Prevzem gradiva po pošti
Naslov za dostavo:
Med podatki člana manjka naslov.
Storitev za pridobivanje naslova trenutno ni dostopna, prosimo, poskusite še enkrat.
S klikom na gumb "V redu" boste potrdili zgoraj izbrano prevzemno mesto in dokončali postopek rezervacije.
S klikom na gumb "V redu" boste potrdili zgoraj izbrano prevzemno mesto in naslov za dostavo ter dokončali postopek rezervacije.
S klikom na gumb "V redu" boste potrdili zgoraj izbrani naslov za dostavo in dokončali postopek rezervacije.
Obvestilo
Trenutno je storitev za avtomatsko prijavo in rezervacijo nedostopna. Gradivo lahko rezervirate sami na portalu Biblos ali ponovno poskusite tukaj kasneje.
Gesla v Splošnem geslovniku COBISS
Izbira mesta prevzema
Gradivo iz matične enote je brezplačno. Če je gradivo na mesto prevzema dostavljeno iz drugih enot, lahko knjižnica to storitev zaračuna.
Mesto prevzema | Status gradiva | Rezervacija |
---|
Rezervacija v teku
Prosimo, počakajte trenutek.
Rezervacija je uspela.
Rezervacija ni uspela.
Rezervacija...
Članska izkaznica:
Mesto prevzema: