-
Priprava sistemov za izražanje steroidne-1-dehidrogenaze v bakteriji Escherichia coli, kvasovki Pichia pastoris in rastlini Solanum lacinatum : magistrsko deloPečar Fonović, UršaMany steroid compounds which are used for local anti-inflammatory treatment possess double bond between C1 and C2 atoms of steroid skeleton. Double bond is introduced by the enzyme ... steroid-1-dehydrogenase. We introduced a gene for steroid-1-dehydrogenase from Nocardia opaca into 3 different expression vectorsČ into pB3 with which we transformed bacteria Escherichia coli, into pPIC9 which we electroporated in yeast Pichia pastoris, and in pBINplus-pUCCABwhich we electroporated in bacteria Agrobacterium tumefaciens. The presence of gene was shown in all expression vectors with PCR reaction, restriction and nucleotide sequence analysis. After introduction of vectors into living organisms we obtained positive clones only in E. coli which showedhighly different phenotype compared with control cells. The cells with target gene grew slowly on plates and formed smaller colonies and in liquid medium sedimented faster than control counterparts. After induction of growingcells no differences in protein expression occured between cells that contain the target gene and cells without it. We prepared nodium cultures of plants Solanum laciniatum and showed that those which were obtained from New Zealand grow more effectively than those from Australia, therefore they are preferred for further transformation experiments. We determined the nucleotideand deduced amino acid sequence of the steroid-1-dehydrogenase gene.Vrsta gradiva - magistrsko deloZaložništvo in izdelava - Ljubljana : [U.Pečar], 2000Jezik - slovenskiCOBISS.SI-ID - 2187028
Avtor
Pečar Fonović, Urša
Drugi avtorji
Štrukelj, Borut
Teme
Steroids |
Biosynthesis |
Oxidoreductases |
Genetics |
Gene transfer |
Gene expression |
Escherichia coli |
Genetics |
Pichia |
Genetics |
Solanaceae |
Genetics |
Steroidi |
Biosinteza |
Oxidoreduktaze |
Genetika |
Gen, prenos |
Gensko izražanje |
Escherichia coli |
Genetika |
Pichia |
Genetika |
Solanaceae |
Genetika |
genetika, biokemična
![loading ... loading ...](themes/default/img/ajax-loading.gif)
Knjižnica | Signatura – lokacija, inventarna št. ... | Status izvoda |
---|---|---|
MF, Centralna medicinska knjižnica, Ljubljana | magistrske naloge PEČAR Urša Priprava IN: 020000898 |
prosto - na dom, čas izposoje: 1 mes. |
![loading ... loading ...](themes/default/img/ajax-loading.gif)
![loading ... loading ...](themes/default/img/ajax-loading.gif)
![loading ... loading ...](themes/default/img/ajax-loading.gif)
Vnos na polico
Trajna povezava
- URL:
Faktor vpliva
Dostop do baze podatkov JCR je dovoljen samo uporabnikom iz Slovenije. Vaš trenutni IP-naslov ni na seznamu dovoljenih za dostop, zato je potrebna avtentikacija z ustreznim računom AAI.
Leto | Faktor vpliva | Izdaja | Kategorija | Razvrstitev | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Baze podatkov, v katerih je revija indeksirana
Ime baze podatkov | Področje | Leto |
---|
Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
---|---|
Pečar Fonović, Urša | 19170 |
Štrukelj, Borut | 07849 |
Izberite prevzemno mesto:
Prevzem gradiva po pošti
Obvestilo
Gesla v Splošnem geslovniku COBISS
Izbira mesta prevzema
Mesto prevzema | Status gradiva | Rezervacija |
---|
Prosimo, počakajte trenutek.