Progesteronski receptorji in heterotrimerni G-proteini v plazemski membrani glive Rhizopus nigricans : magistrsko delo = Progesteron receptors and heterotrimeric G-proteins in the plasma membrane of fungus Rhizopus nigricans : master thesis
Šlajpah, Maja
In filamentous fungus Rhizopus nigricans a steroid hydroxylating multienzyme system is inducible by progesterone and several other steroids. The biologicalsignal carried by progesterone might be ...mediated by receptors, located either in the plasma membrane andžor inside the cell. To elucidate thepossibility that progesterone receptors are present in the plasma membrane,we prepared different membrane fraction (14K, 12K, 40K) by mechanicaldisruption of mycelia and differential centrifugation. The activities of specific mitochondrial and plasma membrane marker enzymes were measured in these fractions. Fraction 40K contained the highest amount of plasma membrane. The protoplast preparation was not succesfull, because of therigid cell wall. In plasma membrane fraction (40K) the progesterone bindingmolecules with Kd= 7x10-8 M and Bmax = 325 fmolžmg were determined by saturation binding analysis. In the 40K fraction the highest basal GTPase activity was detected. The GTPase activity was increased by progesterone in dose response manner. Affnity of progesterone membrane receptors for progesterone and therefore provide an evidence for membrane bound progesteronereceptors coupled to G-proteins. The results obtained with pertussis toxin treated fungal mycelia suggest that progesterone binding molecules in plasma membrane of Rhizopus nigricans are not coupled to GižGo proteins, but rather to Gs like proteins.
Vrsta gradiva - magistrsko delo
Založništvo in izdelava - Ljubljana : [M.Šlajpah], 2000
magistrske naloge ŠLAJPAH Maja Progesteronski IN: 020000902
prosto - na dom, čas izposoje: 1 mes.
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