Akademska digitalna zbirka SLovenije - logo
(UL)
  • Morfometrična analiza sinaptičnih jedrnih skupkov v posamičnih vlaknih hitre in počasne skeletne mišice
    Smrkolj, Tomaž, zdravnik, 1976-
    Introduction and aim: In addition to the differences in gene expression patterns in the synaptic and extrasynaptic regions, the distribution of nucleiis also nonuniform in the mammalian skeletal ... muscle fiber. Myonuclei arepacked at much higher density in the neuromuscular junction (NMJ) region than in the extrajunctional parts of the fiber. Higher concentration of genes in the neuromuscular junction (NMJ) area could theoretically suffice to reach critical concentrations of gene products relevant for maintenance and functioning of the NMJ and might therefore be an additional mechanism contributing to the specialisation of the NMJ part of the fiber. Slow muscle fibers are electromechanically active for longer time periods than fast fibersand the frequency patterns of action potentials are different in both fiber types. This difference might reflect in the distribution of synaptic myonculei and the arrangement of synaptic Schwann cells. In order to elucidatethis question we tested the following hypotheses: 1) The number of synaptic myonuclei is significantly higher in slow fibers than in fast fibers.2) The number of synaptic Schwann celds determined by counting their nuclei is proportional to NMJ area. 3) The reach of the influences of neural trophic factors in the skeletal muscle ftbers does not differ in regard to thefiber type. Methods: Nuclei in single muscle fibers of m.extensor digitorum longus (fast) and m. soleus (slow) were labelled with SYTOX green nuclear acidstain and the endplates with rhodamine-tagged alpha bungarotoxin. The latter undergoes almost irreversible binding to acetylcholine receptors, whichare highly concentrated solely in postsynaptic membrane of NMJ. Images obtained by confocal microscopy on Zeiss confocal microscope were further analysed after three-dimensional reconstruction with image analysing computer software Zeiss LSM 510. (Abstract truncated at 2000 characters).
    Vrsta gradiva - raziskovalno poročilo
    Založništvo in izdelava - Ljubljana : [T. Smrkolj], 2000
    Jezik - slovenski
    COBISS.SI-ID - 2263828

Knjižnica Signatura – lokacija, inventarna št. ... Status izvoda
MF, Centralna medicinska knjižnica, Ljubljana Prešernove naloge
SMRKOLJ Tomaž Morfometrična
IN: 020010371 		prosto - na dom, čas izposoje: 1 mes.
loading ...
loading ...
loading ...

Vnos na polico