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  • Supplementation of rabbit diet with chestnut wood extract : effect on in vitro gas production from three sources of carhohydrates
    Kermauner, Ajda ; Lavrenčič, Andrej, 1965-
    In vitro gas production kinetics of barley grain (BG), sugar beet pulp (SBP) and wheat bran (WB) were determined using three different inocula prepared from caecum contents of 78 day old rabbits. The ... first inocolum was prepared from caecom contents of rabbits fed supplement with 0.5% of chestnut wood extract (CWE, 75% of tannins) in the form of powder (POWD), the second one from caecum contents of rabbit which diet were supplemented with 0.6% of coated CWE (COAT), while the third one from caecum contents not supplemented with CWE (CONT). The cumulated gas production over 60 h of incubation were modelled with Gompertz model. The total potential gas productions (parameter "B" of the Gompertz model) wewre higher when BG and SBP were incubated in CONT (300 and 288 ml for BG and SBP, respectively) than in COAT (270 and 264 ml for BG and SBP, respectively) and POWD (278 and 264 ml for BG and SBP, respectively). For WB the highest parameter " B" was obtained when incubated in POWD; however, the differences between inocula were not statistically significant (>0.05). Maximum fermentation rates (MFR) of BG (21.1 ml/h) and WB (9.7 ml/h) incubated in CONT were greater than those incubated in POWD (16.3 and 8.7 ml/h for BG and WB) or COAT (18.5 and 8.1 ml/h for BG and WB). However, for the greatest MFR was obtained when incubated in POWD (15.1 ml/h), followed by CONT (13.3 ml/h) and COAT (11.8 ml/h). The times of maximum fermentation rate (TMFR) were generally shorter for substrates incubated in POWD (12.6, 7.4 and 8.4 h for BG, SBP and WB, respectivel) than those incubated in CONT and COAT (12.9 and 12.9 f for BG, 9.5 and 9.4 ml for SBP and 9.5 and 8.4 ml for WB, respectivelly). In the first 8 h of fermentation (Gas8), higher (P<0.05) amounts of gas were produced from substrates incubated in POWD (106, 60 and 35 ml for SBP, WB and BG, respectively) than in CONT (88, 46 and 23 ml for SBP, WB and BG, respectively) and COAT (78, 48 and 23 ml for SBP, WB and BG respectively. In conclusion, the use of CWE as powder increases the microbial activity of caecal microorganisms and that the effect in greater with pectinolytic than with amylolytic or hemicellulolytic microbes and/or enzymes. The similar TMFRs and Gas8 of substrates incubated in COAT and CONT suggest that the process of coating was not very efficient as the CWE was not released at the beginning (in first hours) of incubation in the rabbit caecum contents.
    Vir: 9th World Rabbit Congress June 10-13, 2008 Verona (Str. 683-688)
    Vrsta gradiva - prispevek na konferenci
    Leto - 2008
    Jezik - angleški
    COBISS.SI-ID - 2325384

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