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  • Autoimmune reactivity of IgM acquired after oxidation
    Omersel, Jasna ...
    Objectives: Redox-reactive antibodies, mainly of the IgG class, gained a wide area of interest after their autoimmune reactivity was revealed following the application of chemical and physiological ... oxidants. In this study, we examined the susceptibility of IgMs to oxidation and evaluated their binding to the autoantigens important in some autoimmune diseases. Methods: IgM and IgG fractions, isolated from healthy individualsć sera, were oxidized using direct electric current or physiological oxidant hemin. Specificities towards beta-2-glycoprotein I (â2-GPI), cardiolipin (CL), and rheumatoid factor were evaluated with the enzyme-linked immunosorbent assays (ELISAs). Posttranslational modification was investigated by 2,4-dinitrophenylhydrazine reaction. Results: Electrochemically oxidized IgM fractions exhibited altered immunoreactivity - low to medium titers in anti-CL and low positive titers in anti-â2-GPI ELISA but exhibited no rheumatoid factor reactivity. Oxidized IgG and IgM fractions exhibited 2.5- and 5-fold increase in the carbonyl content, respectively. Discussion: An increase in the carbonyl content along with increased immunoreactivity after oxidation suggests modifications of the IgM paratopes. These results point towards possible modifications of native IgMs to their autoimmune state despite the fact that IgMs were less susceptible to oxidation than IgGs. The importance of an individualćs redox status in maintenance of autoimmune reactions was emphasized by in vitro diagnostic tests.
    Vir: Redox report. - ISSN 1351-0002 (Vol. 16, no. 6, 2011, str. 248-256)
    Vrsta gradiva - članek, sestavni del ; neleposlovje za odrasle
    Leto - 2011
    Jezik - angleški
    COBISS.SI-ID - 3180401

    Povezava(-e):

    http://www.ingentaconnect.com/content/maney/rer/2011/00000016/00000006/art00005;jsessionid=dh3ir2h677e1.alexandra
    http://dx.doi.org/10.1179/174329211X13190184351680
    DOI

vir: Redox report. - ISSN 1351-0002 (Vol. 16, no. 6, 2011, str. 248-256)

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