Kinetics of hemolysis induced by equinatoxin, a cytolytic toxin from the sea anemone Actinia equina. : effect of some ions and pH
Maček, Peter ; Lebez, Drago
The kinetics of hemolysis induced by equinatoxin, a cytolytic toxin from the sea anemone Actinia equina, were followed spectrophotometrically by measuring turbidity. The sigmoidal course of hemolysis ...was described using a simple mathematical expression and the parameters of hemolysis were determined. Concentrations of less than 1x10[above]-10 M of equinatoxin caused total hemolysis. Of the ions tested at concentrations of 10mM, Ca[above]2+ stimulated the maximum rate of hemolysis in comparison with the control by a factor of 2.6, Mg[above]2+ by 2.0 and Ba[above]2+ by 1.7 Li+ and EDTA reduced hemolytic activity by 30% while Sr[above]2+ and Mn[above]2+ had no influence. Hemolysis proceeded even in the absence of Ca[above]2+; however, an optimum was determined at Ca[above]2+ concentrations of mM Ca[above]2+, while extremely high concentrations, of a nonphysiological nature, increased the rate of hemolysis to a great extent. Hemolytic activity occured at a pH optimum of 8.8 (8.5 if Ca[above]2+ ions were added). Activity was minimal at pH valus below 6.5.
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