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  • The partial specific volume of ▫$\beta$▫-lactoglobulin A in aqueous urea solutions
    Lapanje, Savo ; Škerjanc, Jože ; Doleček, Valter
    The partial specific volume of ▫$\beta-lactoglobulin$▫ A in 0.02 M NaCl - 0.01M HCl containing different amounts of urea has been determined from density measurements. The partial specific volume ... first increases with urea concentration, reaches a maximum, decreases, reches a minimum, and the increases again. In the interpretation of this behaviour, the binding of urea to the protein and the imperfect atomic packing in native protein molecules have been assumed to be the dominant factors. From dilatometric experiments the differences between the partial molar volume of the protein in 0.02 M Na Cl-0.01 M HCl with and without urea have been obtained. The values of the differences agree satisfactorily with those calculated from the partial specific volume. Furthermore, the volumes as well as their changes reflect the interaction of urea with the protein. Dilatometric expeiments were also performed with the protein in o.o2 M NaCl to which urea was added. Comparison of the obtained results with those in 0.02 M NaCl-0.01 M HCl displays the factthat the partial specific volume id pH-dependent.
    Vir: Croatica chemica acta. - ISSN 0011-1643 (Vol. 43, 1971, str. 65-72)
    Vrsta gradiva - članek, sestavni del
    Leto - 1971
    Jezik - angleški
    COBISS.SI-ID - 5900822

vir: Croatica chemica acta. - ISSN 0011-1643 (Vol. 43, 1971, str. 65-72)

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