(UM)
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Actin coating and compression of fused secretory vesicles are essential for surfactant secretion: a role for Rho, formins and myosin IIMiklavc, Pika ...Secretion of vesicular contents by exocytosis is a fundamental cellular process. Increasing evidence suggests that post-fusion events play an important role in determining the composition and ... quantity of the secretory output. In particular, regulation of fusion pore dilation and closure is considered a key regulator of the post-fusion phase. However, depending on the nature of the cargo, additional mechanisms might be essential to facilitate effective release. We have recently described that in alveolar type II (ATII) cells, lamellar bodies (LBs), which are secretory vesicles that store lung surfactant, are coated with actin following fusion with the plasma membrane. Surfactant, a lipoprotein complex, does not readily diffuse out of fused LBs following opening and dilation of the fusion pore. Using fluorescence microscopy, atomic force microscopy and biochemical assays, we present evidence that actin coating and subsequent contraction of the actin coat is essential to facilitate surfactant secretion. Latrunculin B prevents actin coating of fused LBs and inhibits surfactant secretion almost completely. Simultaneous imaging of the vesicle membrane and the actin coat revealed that contraction of the actin coat compresses the vesicle following fusion. This leads to active extrusion of vesicle contents. Initial actin coating of fused vesicles is dependent on activation of Rho and formin-dependent actin nucleation. Actin coat contraction is facilitated by myosin II. In summary, our data suggest that fusion pore opening and dilation it self is not sufficient for release of bulky vesicle cargos and that active extrusion mechanisms are required.Vir: Journal of cell science. - ISSN 0021-9533 (iss. 11, Vol. 125, 2012, str. 2765-2774)Vrsta gradiva - članek, sestavni delLeto - 2012Jezik - angleškiCOBISS.SI-ID - 75511041
Avtor
Miklavc, Pika |
Hecht, Elena |
Hobi, Nina |
Wittekindt, Oliver H. |
Dietl, Paul |
Kranz, C. |
Frick, Manfred
Teme
Actin |
Exocytosis |
Formin |
Lamellar body |
Myosin |
Rho
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Leto | Faktor vpliva | Izdaja | Kategorija | Razvrstitev | ||||
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Baze podatkov, v katerih je revija indeksirana
Ime baze podatkov | Področje | Leto |
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Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
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Miklavc, Pika | 21409 |
Hecht, Elena | ![]() |
Hobi, Nina | ![]() |
Wittekindt, Oliver H. | ![]() |
Dietl, Paul | ![]() |
Kranz, C. | ![]() |
Frick, Manfred | ![]() |
Vir: Osebne bibliografije
in: SICRIS
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