To define sensitivity and specificity of Vitek 2 MICs as phenotypic screening method for carbapenemase-producing . We determined Vitek 2 MICs of antipseudomonal antimicrobials in 130 unrelated carbapenemase-producing and 129 carbapenemase-negative isolates within a Dutch carbapenemase-surveillance database. We calculated test characteristics of single and combined antimicrobial MICs for carbapenemase production. Vitek 2 MIC above epidemiological cutoff of both imipenem and tobramycin or ciprofloxacin and tobramycin displayed a sensitivity of 96.2% and specificity of 89.6% for carbapenemase production in . Vitek 2 MIC> epidemiological cut-off values seem sensitive and specific as a phenotypic screening strategy for carbapenemase-producing . Combining imipenem and tobramycin or ciprofloxacin and tobramycin performed best as a screening strategy for defining which isolates should undergo confirmatory tests for carbapenemase production.