In the current study, a novel strategy using cyclic shifting of temperature was developed for simultaneous saccharification and fermentation (SSF) for bioethanol production from rice straw. The in-situ cellulase production, saccharification and fermentation was carried out using P. janthinellum and S. cerevisiae. Bioethanol titer of 14.98 g/l was obtained using base followed by acid pretreated rice straw by employing the cyclic shifting of temperature strategy "30 °C for 2 h to 40 °C for 2 h". The holding time was further tuned to increase the productivity and the tuned condition 30 °C(1.7 h) - 40 °C(2 h) improved the bioethanol titer to 15.9 g/l. Using this strategy, resulted 5.1-fold and 2.8-fold increment of bioethanol production compared to known approaches, SSF at mutual optimum temperature and prolong prehydrolysis followed by fermentation respectively. The application of cyclic shifting of temperature strategy can unleash a great potential in enhancing the yield and efficiency for a sustainable lignocellulosic bioethanol production.