E-viri
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Genzel, Yvonne; Fischer, Marlies; Olmer, Ruth Maria; Schäfer, Bastian; Best, Claudia; König, Susanne; Hundt, Boris; Reichl, Udo
Cell Technology for Cell ProductsBook Chapter
A serum-free process for influenza virus vaccine production (equine and human) in roller bottles and microcarrier systems in 5L-stirred tank and 2Lwave bioreactor (Cytodex 1) is described. MDCK cells were adapted from growth in serum containing GMEM medium to serum-free Ex-Cell MDCK medium. Virus titers of 2.0–2.9 log HA units/ 100 μL were obtained. Omission of the medium exchange before infection has clearly simplified the process.
