E-viri
Recenzirano
Odprti dostop
-
Shi, Junchao; Zhang, Yunfang; Tan, Dongmei; Zhang, Xudong; Yan, Menghong; Zhang, Ying; Franklin, Reuben; Shahbazi, Marta; Mackinlay, Kirsty; Liu, Shichao; Kuhle, Bernhard; James, Emma R; Zhang, Liwen; Qu, Yongcun; Zhai, Qiwei; Zhao, Wenxin; Zhao, Linlin; Zhou, Changcheng; Gu, Weifeng; Murn, Jernej; Guo, Jingtao; Carrell, Douglas T; Wang, Yinsheng; Chen, Xuemei; Cairns, Bradley R; Yang, Xiang-Lei; Schimmel, Paul; Zernicka-Goetz, Magdalena; Cheloufi, Sihem; Zhang, Ying; Zhou, Tong; Chen, Qi
Nature cell biology, 04/2021, Letnik: 23, Številka: 4Journal Article
Although high-throughput RNA sequencing (RNA-seq) has greatly advanced small non-coding RNA (sncRNA) discovery, the currently widely used complementary DNA library construction protocol generates biased sequencing results. This is partially due to RNA modifications that interfere with adapter ligation and reverse transcription processes, which prevent the detection of sncRNAs bearing these modifications. Here, we present PANDORA-seq (panoramic RNA display by overcoming RNA modification aborted sequencing), employing a combinatorial enzymatic treatment to remove key RNA modifications that block adapter ligation and reverse transcription. PANDORA-seq identified abundant modified sncRNAs-mostly transfer RNA-derived small RNAs (tsRNAs) and ribosomal RNA-derived small RNAs (rsRNAs)-that were previously undetected, exhibiting tissue-specific expression across mouse brain, liver, spleen and sperm, as well as cell-specific expression across embryonic stem cells (ESCs) and HeLa cells. Using PANDORA-seq, we revealed unprecedented landscapes of microRNA, tsRNA and rsRNA dynamics during the generation of induced pluripotent stem cells. Importantly, tsRNAs and rsRNAs that are downregulated during somatic cell reprogramming impact cellular translation in ESCs, suggesting a role in lineage differentiation.
Avtor
Vnos na polico
Trajna povezava
- URL:
Faktor vpliva
Dostop do baze podatkov JCR je dovoljen samo uporabnikom iz Slovenije. Vaš trenutni IP-naslov ni na seznamu dovoljenih za dostop, zato je potrebna avtentikacija z ustreznim računom AAI.
Leto | Faktor vpliva | Izdaja | Kategorija | Razvrstitev | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Baze podatkov, v katerih je revija indeksirana
Ime baze podatkov | Področje | Leto |
---|
Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
---|
Vir: Osebne bibliografije
in: SICRIS
To gradivo vam je dostopno v celotnem besedilu. Če kljub temu želite naročiti gradivo, kliknite gumb Nadaljuj.