E-viri
Recenzirano
Odprti dostop
-
Pichler, Martin; Rodriguez-Aguayo, Cristian; Nam, Su Youn; Dragomir, Mihnea Paul; Bayraktar, Recep; Anfossi, Simone; Knutsen, Erik; Ivan, Cristina; Fuentes-Mattei, Enrique; Lee, Sang Kil; Ling, Hui; Catela Ivkovic, Tina; Huang, Guoliang; Huang, Li; Okugawa, Yoshinaga; Katayama, Hiroyuki; Taguchi, Ayumu; Bayraktar, Emine; Bhattacharya, Rajat; Amero, Paola; He, William Ruixian; Tran, Anh M; Vychytilova-Faltejskova, Petra; Klec, Christiane; Bonilla, Diana L; Zhang, Xinna; Kapitanovic, Sanja; Loncar, Bozo; Gafà, Roberta; Wang, Zhihui; Cristini, Vittorio; Hanash, Samir M; Bar-Eli, Menashe; Lanza, Giovanni; Slaby, Ondrej; Goel, Ajay; Rigoutsos, Isidore; Lopez-Berestein, Gabriel; Calin, George Adrian
Gut, 10/2020, Letnik: 69, Številka: 10Journal Article
To investigate the function of a novel primate-specific long non-coding RNA (lncRNA), named FLANC, based on its genomic location (co-localised with a pyknon motif), and to characterise its potential as a biomarker and therapeutic target. FLANC expression was analysed in 349 tumours from four cohorts and correlated to clinical data. In a series of multiple in vitro and in vivo models and molecular analyses, we characterised the fundamental biological roles of this lncRNA. We further explored the therapeutic potential of targeting FLANC in a mouse model of colorectal cancer (CRC) metastases. FLANC, a primate-specific lncRNA feebly expressed in normal colon cells, was significantly upregulated in cancer cells compared with normal colon samples in two independent cohorts. High levels of FLANC were associated with poor survival in two additional independent CRC patient cohorts. Both in vitro and in vivo experiments demonstrated that the modulation of FLANC expression influenced cellular growth, apoptosis, migration, angiogenesis and metastases formation ability of CRC cells. In vivo pharmacological targeting of FLANC by administration of 1,2-dioleoyl-sn-glycero-3-phosphatidylcholine nanoparticles loaded with a specific small interfering RNA, induced significant decrease in metastases, without evident tissue toxicity or pro-inflammatory effects. Mechanistically, FLANC upregulated and prolonged the half-life of phosphorylated STAT3, inducing the overexpression of VEGFA, a key regulator of angiogenesis. Based on our findings, we discovered, FLANC as a novel primate-specific lncRNA that is highly upregulated in CRC cells and regulates metastases formation. Targeting primate-specific transcripts such as FLANC may represent a novel and low toxic therapeutic strategy for the treatment of patients.
Avtor
Vnos na polico
Trajna povezava
- URL:
Faktor vpliva
Dostop do baze podatkov JCR je dovoljen samo uporabnikom iz Slovenije. Vaš trenutni IP-naslov ni na seznamu dovoljenih za dostop, zato je potrebna avtentikacija z ustreznim računom AAI.
Leto | Faktor vpliva | Izdaja | Kategorija | Razvrstitev | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Baze podatkov, v katerih je revija indeksirana
Ime baze podatkov | Področje | Leto |
---|
Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
---|
Vir: Osebne bibliografije
in: SICRIS
To gradivo vam je dostopno v celotnem besedilu. Če kljub temu želite naročiti gradivo, kliknite gumb Nadaljuj.