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Rooney, John P; Ryde, Ian T; Sanders, Laurie H; Howlett, Evan H; Colton, Meryl D; Germ, Kaylyn E; Mayer, Greg D; Greenamyre, J Timothy; Meyer, Joel N
Methods in molecular biology, 01/2015, Letnik: 1241Journal Article
Mitochondrial DNA (mtDNA) copy number is a critical component of overall mitochondrial health. In this chapter, we describe methods for isolation of both mtDNA and nuclear DNA (nucDNA) and measurement of their respective copy numbers using quantitative PCR. Methods differ depending on the species and cell type of the starting material and availability of specific PCR reagents.
