E-viri
Recenzirano
Odprti dostop
-
KURU, Kevser; AYDEMİR, Tülin
Turkish journal of chemistry, 2003, Letnik: 27, Številka: 1Journal Article
Catalase plays a major role in the protection of tissues from the toxic effects of $H_2O_2$ and partially reduced oxygen species. A nearly 136-fold enzyme purification was obtained from chicken erythrocyte by acetone precipitation, ethanol-chloroform treatment, CM-cellulose and Sephadex G-200 chromatography. The specific activity of purified enzyme was 42,556 U/mg. The molecular weight of the native chicken erythrocyte catalase was estimated at 240 kDa by gel filtration. SDS-gel electrophoresis results indicated that chicken erythrocyte catalase consists of four apparently identical subunits, with a molecular weight of around 57.5 kDa. The optical spectrum of the purified enzyme shows a Soret band at 406 nm, which is the characteristic for the heme group. Dithionite treatment of the enzyme resulted in the reduction of enzyme.The Km of chicken erythrocyte catalase was 33 mM $H_2O_2$. The maximal activity of catalase was observed between pH 6.0 and 8.0. Enzyme activity was stable at temperatures between 10 and 30 C. The activity of purified catalase was inhibited by azide, cyanide, $\beta$ -mercaptoethanol, dithiotreitol (DTT) and iodoacetamide.
![loading ... loading ...](themes/default/img/ajax-loading.gif)
Vnos na polico
Trajna povezava
- URL:
Faktor vpliva
Dostop do baze podatkov JCR je dovoljen samo uporabnikom iz Slovenije. Vaš trenutni IP-naslov ni na seznamu dovoljenih za dostop, zato je potrebna avtentikacija z ustreznim računom AAI.
Leto | Faktor vpliva | Izdaja | Kategorija | Razvrstitev | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Baze podatkov, v katerih je revija indeksirana
Ime baze podatkov | Področje | Leto |
---|
Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
---|
Vir: Osebne bibliografije
in: SICRIS
To gradivo vam je dostopno v celotnem besedilu. Če kljub temu želite naročiti gradivo, kliknite gumb Nadaljuj.