Akademska digitalna zbirka SLovenije - logo
E-viri
Celotno besedilo
Recenzirano
  • Low‐scale syringe‐made synt...
    Brož, Břetislav; Tureček, František; Marek, Aleš

    Journal of labelled compounds & radiopharmaceuticals, 10/2022, Letnik: 65, Številka: 12
    Journal Article

    Fast and reasonable low‐scale (200 nmol) syringe‐made synthesis of 15 N‐labeled oligonucleotides representing DNA trinucleotide codons is communicated. All codons were prepared by solid‐phase controlled pore glass synthesis column technique via the phosphoramidite method. Twenty‐four labeled oligonucleotides covering the DNA genetic code alphabet were prepared using commercially available reagents and affordable equipment in a reasonably short period of time, with acceptable yields and purity for direct applications in mass spectrometry.