The scaffolding protein Yotiao is a member of a large family of protein A-kinase anchoring proteins with important roles in the organization of spatial and temporal signaling. In heart, Yotiao directly associates with the slow outward potassium ion current (IKs) and recruits both PKA and PP1 to regulate IKs phosphorylation and gating. Human mutations that disrupt IKs-Yotiao interaction result in reduced PKA-dependent phosphorylation of the IKs subunit KCNQ1 and inhibition of sympathetic stimulation of IKs, which can give rise to long-QT syndrome. We have previously identified a subset of adenylyl cyclase (AC) isoforms that interact with Yotiao, including AC1–3 and AC9, but surprisingly, this group did not include the major cardiac isoforms AC5 and AC6. We now show that either AC2 or AC9 can associate with KCNQ1 in a complex mediated by Yotiao. In transgenic mouse heart expressing KCNQ1-KCNE1, AC activity was specifically associated with the IKs-Yotiao complex and could be disrupted by addition of the AC9 N terminus. A survey of all AC isoforms by RT-PCR indicated expression of AC4–6 and AC9 in adult mouse cardiac myocytes. Of these, the only Yotiao-interacting isoform was AC9. Furthermore, the endogenous IKs-Yotiao complex from guinea pig also contained AC9. Finally, AC9 association with the KCNQ1-Yotiao complex sensitized PKA phosphorylation of KCNQ1 to β-adrenergic stimulation. Thus, in heart, Yotiao brings together PKA, PP1, PDE4D3, AC9, and the IKs channel to achieve localized temporal regulation of β-adrenergic stimulation. Background: In heart, the A-kinase anchoring protein (AKAP) Yotiao anchors the KCNQ1 subunit of the slow outward potassium ion current (IKs). Results: Adenylyl cyclase type 9 (AC9) is associated with the IKs complex and sensitizes PKA phosphorylation of KCNQ1 by isoproterenol. Conclusion: Yotiao facilitates complex formation between AC9 and IKs in heart. Significance: Sympathetic regulation of IKs is important for repolarization in heart.