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Wu, Aimin; Lian, Boying; Hao, Pengbo; Fu, Xiaokang; Zhang, Meng; Lu, Jianhua; Ma, Liang; Yu, Shuxun; Wei, Hengling; Wang, Hantao
The Plant journal : for cell and molecular biology, February 2024, 2024-Feb, 2024-02-00, 20240201, Letnik: 117, Številka: 3Journal Article
SUMMARY Xyloglucan, an important hemicellulose, plays a crucial role in maintaining cell wall structure and cell elongation. However, the effects of xyloglucan on cotton fiber development are not well understood. GhMUR3 encodes a xyloglucan galactosyltransferase that is essential for xyloglucan synthesis and is highly expressed during fiber elongation. In this study, we report that GhMUR3 participates in cotton fiber development under the regulation of GhMYB30. Overexpression GhMUR3 affects the fiber elongation and cell wall thickening. Transcriptome showed that the expression of genes involved in secondary cell wall synthesis was prematurely activated in OE‐MUR3 lines. In addition, GhMYB30 was identified as a key regulator of GhMUR3 by Y1H, Dual‐Luc, and electrophoretic mobility shift assay (EMSA) assays. GhMYB30 directly bound the GhMUR3 promoter and activated GhMUR3 expression. Furthermore, DAP‐seq of GhMYB30 was performed to identify its target genes in the whole genome. The results showed that many target genes were associated with fiber development, including cell wall synthesis‐related genes, BR‐related genes, reactive oxygen species pathway genes, and VLCFA synthesis genes. It was demonstrated that GhMYB30 may regulate fiber development through multiple pathways. Additionally, GhMYB46 was confirmed to be a target gene of GhMYB30 by EMSA, and GhMYB46 was significantly increased in GhMYB30‐silenced lines, indicating that GhMYB30 inhibited GhMYB46 expression. Overall, these results revealed that GhMUR3 under the regulation of GhMYB30 and plays an essential role in cotton fiber elongation and secondary wall thickening. Additionally, GhMYB30 plays an important role in the regulation of fiber development and regulates fiber secondary wall synthesis by inhibiting the expression of GhMYB46. Significance Statement In this study, GhMUR3 was identified and cloned in Gossypium hirsutum, confirming that it affects fiber elongation and secondary wall thickening and plays an important role in fiber development. In addition, GhMYB30 was screened as an upstream transcription factor to directly regulate GhMUR3 expression. Combined with the results of DAP‐seq, the possibility of GhMYB30 affecting fiber development through multiple pathways was expounded. This study will provide a new research direction for improving the quality of cotton fiber.
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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