E-viri
Recenzirano
-
Makoni, Nyasha J.; Garrad, Evan C.; Redzic, Adela; Nichols, Michael R.
Biotechnology progress, July/August 2021, Letnik: 37, Številka: 4Journal Article
The NLRP3 inflammasome is a key intracellular component of the innate immune response. It is a three‐protein complex essential for the production of mature interleukin 1‐β. The complex, which is comprised of three proteins, NLRP3, ASC, and pro‐caspase‐1, has been implicated in the physiological response to pathogenic elements of cardiovascular disease and Alzheimer's disease. Investigations into the properties of the three proteins can be aided by larger‐scale recombinant expression to produce adequate amounts. In the current study, a variety of NLRP3 inflammasome proteins were expressed in the ExpiCHO‐S mammalian cell system with a particular focus on ASC. ASC fusion proteins with glutathione‐S transferase, maltose‐binding protein, and SUMO increased solubility and aided in determining the stability and oligomerization propensity of individual ASC domains and full‐length ASC. ASC oligomerization was highly sensitive to protein concentration, ionic strength, and mutation. These observations provided strategic ways to enhance protein purification and characterize ASC oligomerization. The ExpiCHO‐S expression system consistently produced high‐yield recombinant NLRP3 inflammasome proteins which led to a further understanding of ASC oligomerization.
![loading ... loading ...](themes/default/img/ajax-loading.gif)
Vnos na polico
Trajna povezava
- URL:
Faktor vpliva
Dostop do baze podatkov JCR je dovoljen samo uporabnikom iz Slovenije. Vaš trenutni IP-naslov ni na seznamu dovoljenih za dostop, zato je potrebna avtentikacija z ustreznim računom AAI.
Leto | Faktor vpliva | Izdaja | Kategorija | Razvrstitev | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Baze podatkov, v katerih je revija indeksirana
Ime baze podatkov | Področje | Leto |
---|
Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
---|
Vir: Osebne bibliografije
in: SICRIS
To gradivo vam je dostopno v celotnem besedilu. Če kljub temu želite naročiti gradivo, kliknite gumb Nadaljuj.