In several studies, these markers have been studied either singly or combined in different geographical locations4,5, but there are limited studies from Southeast Asia. ...with a view to determine the circulating dengue serotype(s) and to study the inter-relationship of all the above markers, this observational study was initiated in a tertiary care hospital of Dhaka city, the capital of Bangladesh from 2013 to 2016. Dengue virus infection with DEN-1 has been reported from China, Malaysia and India in 2005, 2010 and 2014, respectively11,12,13,14. ...circulation of DEN-1 in Dhaka city may have a link with any of these geographical areas. ...the variations in viral load of each serotype may differ from geographical area to area in which dengue genotype may have a role.