Dermatophytosis is a common dermatological infection, affecting humans, pets, domestic ruminants and wild animals. It is a cause for concern as it represents a risk to public and animal health. The aim of this study was to describe the occurrence of dermatophyte infection in wild mammals (road-killed and hunted) in Portugal. Fungal isolation was performed on the fur and scales of 101 wild mammals. Based on the observation of microstructures and colony morphology, fungal isolates were identified to the genus level. Dermatophytes were found in two of the 10 studied species: 3/51 (5.9%; 95% confidence interval CI: 0.0–12.3%) red fox (Vulpes vulpes), and 5/19 (2.6%; 95% CI: 0.0–9.8%) Egyptian mongoose (Herpestes ichneumon). These species represent different taxonomic families: Canidae and Viverridae and it is therefore important to infer the role of infection in these animals studied for dermatophytosis. Dermatofitoza je česta dermatološka infekcija koja pogađa ljude, kućne ljubimce, domaće glodavce i divlje životinje. Dermatofiti zadaju brigu i predstavljaju rizik za javno zdravlje i zdravlje životinja. Cilj ove studije bio je opisati pojavnost infekcije dermatofitima u divljih sisavaca u Portugalu, u životinja koje su nastradale u prometu i uhvaćenih divljih sisavaca. Izolacija gljivica obavljena je u krznu i ljuskama 101 divljeg sisavca. Na temelju promatranja mikrostruktura i morfologije kolonije, izolati gljivica su identificirani na razini roda. Dermatofiti su pronađeni u dvije od 10 proučavanih vrsta: 3/51 (5,9 %; 95 % interval pouzdanosti CI: 0,0-12,3 %) crvene lisice (Vulpes vulpes) i 5/19 (2,6 %; 95 % CI: 0,0-9,8 %) egipatskih mungosa (Herpestes ichneumon). Ove vrste predstavljaju različite taksonomske obitelji: Canidae i Viverridae i stoga je važno donijeti zaključak o ulozi infekcije u ovih životinja ispitanih na dermatofitozu.