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  • Prevalence of bovine mastit...
    Bertolini, A.B.; Prado, A.M.; Thyssen, P.J.; Souza Ribeiro Mioni, M.; Gouvea, F.L.R.; Silva Leite, D.; Langoni, H.; Figueiredo Pantoja, J.C.; Rall, V.M.; Guimarães, F.F.; Joaquim, S.F.; Guerra, S.T.; Hernandes, R.T.; Lucheis, S.B.; Ribeiro, M.G.

    Letters in applied microbiology, November 2022, 2022-11-01, 20221101, Letnik: 75, Številka: 5
    Journal Article

    Diptera (Insecta) are able to transmit approximately 200 pathogenic microorganisms to humans and animals, causing more than 65 diseases, including bovine mastitis, which constitutes a major cause of economic losses in the dairy industry. In this scenario, 217 adult specimens of Diptera were collected from nine farms and identified to the family and/or species level. Of the 11 families recorded, Muscidae was the most prevalent (152 out of 217; 70%). All Diptera specimens were subjected to microbiological culture using conventional and selective media, and isolates were then identified at the species level by mass spectrometry. In total, 275 microorganisms were identified, with a predominance of pathogens related to environmental bovine mastitis (166/275 = 60·4%), that is, Enterococcus species (70/275 = 25·4%) and Escherichia coli (49/275 = 17·8%). Nontraditional agents related to bovine mastitis (called miscellaneous) were detected in 28% (77/275), as well as microorganisms with well‐known zoonotic behaviour (e.g. Bacillus cereus). This is study contributes with knowledge of diversity of microorganisms carried by Diptera in the dairy environment, including pathogens associated with environmental and contagious bovine mastitis, and agents with human relevance. To our knowledge, a three‐part chromogenic selective medium used to microbial culture of milk on farms was used for the first time to identification of pathogens in Diptera. Significance and Impact of the Study: The high diversity of Diptera (Insecta) species (>157 000) worldwide, feeding habits, and the access to contaminated environments, make these insects potential vector‐borne of more than 200 pathogens to humans and animals. Here, 217 adult specimens of Diptera were collected in the environment of nine dairy farms with a history of clinical mastitis. From these, 275 microorganisms were identified, with a predominance of pathogens related to environmental bovine mastitis (Enterococcus spp. and Escherichia coli), in addition to agents with well‐known zoonotic nature (e.g. Bacillus cereus), indicating that control of this insect in dairy farms pose relevance in human and animal health.