Purpose To determine the utility of Split‐Window optical coherence tomography OCT (SW‐OCT) biometry in measuring ocular axial dimensions as well as imaging the intraocular lens (IOL) and posterior capsule in pseudophakic eyes. Methods Sixty‐nine pseudophakic eyes of 69 subjects were enrolled in the study. The results of SW‐OCT biometry implemented in the SD OCT device for posterior and anterior segment imaging (REVO NX, Optopol Technology) were compared with those obtained with the SS‐OCT‐based biometer IOLMaster 700 (Carl Zeiss Meditec). Differences in measurement values between the two biometers were determined using the paired t‐test. Agreement was assessed through intraclass correlation coefficients (ICC) and Bland–Altman plots. Results The correlation between measurements obtained with SW‐OCT and SS‐OCT was very high (ICC for: axial length (AL) = 1.000; anterior chamber depth (ACD) = 0.997; IOL thickness (IOL LT) = 0.997; central corneal thickness (CCT) = 0.987). The mean AL measurement difference was 0.003 ± 0.021 mm (the 95% LoA ranged from −0.04 to 0.05); the mean ACD difference was −0.009 ± 0.025 mm (95% LoA, −0.06 to 0.04); mean LT difference was 0.001 ± 0.021 mm (95% LoA, −0.04 to 0.04); and mean CCT difference was 1.4 ± 5.4 μm (95% LoA, −9 to 12). Conclusion The study shows small, non‐significant differences between the biometric measurements obtained with REVO NX SW‐OCT and IOLMaster 700 SS‐OCT in pseudophakic eyes. However, SW‐OCT offered significantly lower ACD and LT measurement failure rates. With high‐resolution imaging, SW‐OCT enables accurate assessment of IOL position relative to the posterior capsule and visualization of capsular fibrosis.