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Konopsky, Valery N; Alieva, Elena V
Analytical chemistry, 06/2007, Letnik: 79, Številka: 12Journal Article
We present a new optical biosensor technique based on registration of dual optical s-polarized modes on a photonic crystal surface. The simultaneous registration of two optical surface waves with different evanescent depths from the same surface spot permits the segregation of the volume and the surface contributions from an analyte, while the absence of metal damping permits an increase in the propagation length of the optical surface waves and the sensitivity of the biosensor. Our technique was tested with the binding of biotin molecules to a streptavidin monolayer that has been detected with signal/noise ratio of ∼15 at 1-s signal accumulation time. The detection limit is ∼20 fg of the analyte on the probed spot of the surface.
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