•Laser method for synthesis of bimetallic materials for enzyme-free microbiosensors.•Relationship between the structure of complexes and the properties of microbiosensors is shown.•The gap in knowledge about the structure of bimetal wine complexes has been filled. The use of laser methods for synthesizing new materials for non-enzymatic electrochemical microsensors for express analysis of biological fluids is a promising scientific question. Laser-induced chemical liquid phase deposition is the laser deposition of invisible miniature bimetallic deposits from aqueous solutions of salts and complexes of transition metals. Bimetallic complexes containing two transition metal cations in the shell of one ligand are of increased interest. There is an information gap in this area even for ligands such as tartrate. The studies carried out make it possible to obtain new information on the structure of bimetallic tartaric complexes in solutions containing simultaneously copper, nickel, silver, iron, and cobalt.