E-viri
Recenzirano
Odprti dostop
-
Cuthbert, Richard J.; Fragkakis, Evangelos M.; Dunsmuir, Robert; Li, Zhi; Coles, Mark; Marzo‐Ortega, Helena; Giannoudis, Peter V.; Jones, Elena; El‐Sherbiny, Yasser M.; McGonagle, Dennis
Arthritis & rheumatology, September 2017, Letnik: 69, Številka: 9Journal Article
Objective Group 3 innate lymphoid cells (ILC3s) play a pivotal role in barrier tissues such as the gut and the skin, two important sites of disease in spondyloarthritis (SpA). This study was undertaken to investigate whether normal or injured human enthesis, a key target tissue in early SpA, harbors ILC3s in entheseal soft tissue and adjacent perientheseal bone. Methods Interspinous ligament and spinous process bone from donors with no systemic inflammatory disease were collected, enzymatically digested, and immunophenotyped. The immunologic profile of entheseal cells was examined, and the transcriptional profile of sorted ILC3s was compared to that of ILC3s isolated from SpA synovial fluid (SF). To assess the ability of entheseal tissue to produce interleukin‐17 (IL‐17) and IL‐22, entheseal digests were stimulated with IL‐23 and IL‐1β. Osteoarthritic and ruptured Achilles tendon tissue was examined histologically. Results The proportion of ILCs in human entheseal soft tissue was higher than that in peripheral blood (P = 0.008); entheseal soft tissue and perientheseal bone both had a higher proportion of NKp44+ ILC3s (P = 0.001 and P = 0.043, respectively). Studies of retinoic acid receptor–related orphan nuclear receptor γt (RORγt), STAT3, and IL‐23 receptor transcript expression validated the entheseal ILC3 phenotype. Cytokine transcript expression was similar in ILC3s isolated from enthesis and from SpA SF. Stimulation of normal entheseal digests with IL‐23/IL‐1β led to up‐regulation of IL‐17A transcript, and histologic examination of injured/damaged entheses revealed the presence of RORγt‐expressing cells. Conclusion This work shows that human enthesis harbors a resident population of ILC3s, with the potential to participate in the pathogenesis of SpA.
![loading ... loading ...](themes/default/img/ajax-loading.gif)
Vnos na polico
Trajna povezava
- URL:
Faktor vpliva
Dostop do baze podatkov JCR je dovoljen samo uporabnikom iz Slovenije. Vaš trenutni IP-naslov ni na seznamu dovoljenih za dostop, zato je potrebna avtentikacija z ustreznim računom AAI.
Leto | Faktor vpliva | Izdaja | Kategorija | Razvrstitev | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Baze podatkov, v katerih je revija indeksirana
Ime baze podatkov | Področje | Leto |
---|
Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
---|
Vir: Osebne bibliografije
in: SICRIS
To gradivo vam je dostopno v celotnem besedilu. Če kljub temu želite naročiti gradivo, kliknite gumb Nadaljuj.