The aim of this study is to perform phylogenetic grouping, to detect the virulence associated genes in Escherichia coli strains recovered from the urinary tract infections of dogs and cats, and to determine the antibiotic susceptibility profiles, as well. The urine samples from the animals showing clinical signs of urinary tract infection examined for the occurrence of E. coli. Phylogenetic classification of the isolates was performed according to the existence of chuA, yjaA and TSPE4.C2 genes. The isolates were also examined for the presence of uropathogenecity-associated genes, such as, fimH, sfa1, iut, fyu, hly, cnf-1, papG and for the diversity of the papG allelles. Finally, the antimicrobial susceptibility profiles were examined. E. coli was isolated from 11 (6.08%) of 181 samples. Two isolates belonged to group A-2, while the remaining isolates belonged to group B-2. hylA was detected in 2 (25%); sfa1, cnf1, papG and papGIII were detected in 3 (37.5%); iutA was detected in 4 (50%);  fyuA was detected in 6 (75%); and fimH was detected in all of the isolates. Most of the isolates were resistant to ampicillin while one isolate also showed multidrug resistance. The isolation rate of E. coli was low in this study, however, we had a brief data about the phylo-groups and virulence factors of canine and feline uropathogenic strains.