Dominant arbuscular mycorrhizal (AM) fungi spores, the bacterium Rhizobium sp. along with the fungus Trichoderma viride, were isolated from the rhizosphere of Acacia catechu and mass-produced in laboratory. The co-inoculation effect of Glomus mosseae, Glomus fasciculatum, mixed AM (Glomus spp. (except G. mosseae, G. fasciculatum) with Acaulospora spp., Sclerocystis spp. and Gigaspora spp.), Rhizobium sp. and Trichoderma viride on the growth of A. catechu seedlings was studied. All inoculated seedlings showed improved seedling growth compared to the control. Inoculated seedlings had a pronounced effect on all growth characteristics such as height, fresh and dry weight of roots and shoots, AM spore count, per cent mycorrhizal colonization in roots and root nodule number in comparison with uninoculated seedlings. Phosphorus uptake was also higher in inoculated seedlings than in the control.