The purpose of this study was to investigate the possible influence of different emulsification methods and formulation composition such as emulsifier type, emulsifier concentration, liquid oil type and film-forming agents on the matrix carrier and its comprehensive influence on the UV-protective property of UV-filters. Different particle size of sunscreen emulsions were prepared by different emulsification methods with the same concentration on UVA filters only or combined UVB filters. The size and size distribution of the droplets were analyzed by dynamic light scattering, and the UV protective property was evaluated by UV-Vis absorption spectroscopy in solution and 3 M tape methods. The results showed the synergistic UV protection effect could be realized when the particle size of the matrix carrier was equivalent with the wavelength of the ultraviolet light at the same UV filters dosage. In addition, liquid oil also affected the UV protection performance of sunscreen by the intermiscibility and homogeneity with the UV filters. The addition of film-forming polymers made all system had good UV absorption properties.