E-viri
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Wu, Chao; Wagner, Nicole D.; Moyle, Austin B.; Feng, Annie; Sharma, Nitin; Stubbs, Sarah H.; Donahue, Callie; Davey, Robert A.; Gross, Michael L.; Leung, Daisy W.; Amarasinghe, Gaya K.
Journal of molecular biology, 10/2023, Letnik: 435, Številka: 20Journal Article
•In vitro purified Ebola eNP0VP35 spontaneously phase separates, likely seeding early stages of viral IB assembly.•Construct screening illuminates key interactions within phase separated droplets.•Ebola IBs are composed of eNP0VP35 complexes and other key viral and host components.•Perturbation of eNP0VP35 complex reduces viral IB formation and viral infection. Viral inclusion bodies (IBs) are potential sites of viral replication and assembly. How viral IBs form remains poorly defined. Here we describe a combined biophysical and cellular approach to identify the components necessary for IB formation during Ebola virus (EBOV) infection. We find that the eNP0VP35 complex containing Ebola nucleoprotein (eNP) and viral protein 35 (eVP35), the functional equivalents of nucleoprotein (N) and phosphoprotein (P) in non-segmented negative strand viruses (NNSVs), phase separates to form inclusion bodies. Phase separation of eNP0VP35 is reversible and modulated by ionic strength. The multivalency of eVP35, and not eNP, is also critical for phase separation. Furthermore, overexpression of an eVP35 peptide disrupts eNP0VP35 complex formation, leading to reduced frequency of IB formation and limited viral infection. Together, our results show that upon EBOV infection, the eNP0VP35 complex forms the minimum unit to drive IB formation and viral replication.
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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in: SICRIS
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