Recently electrospun nanofibers have been fabricated from biodegradable polymers, which received attention due to their applications in various fields such as packaging nanomaterials. In this study, Aloe vera nanofibers which contained Pseudomonas sp . bacteriophages were fabricated using electrospinning machine. The nanofibers were characterized ultra-structurally using atomic force microscopy (AFM). Mechanical stress of the nanofibers was measured using tensile test system (TTS). Thermodynamical characteristics of the nanofibers were analyzed via s imultaneous thermal analyzer (STA). Moreover, the biofunction of the electrospun nanofibers was checked via microbial experiments. The results demonstrated significant changes in the morphology of aloe vera nanofibers after addition of the phages. The roughness parameters and topographical ultra-structures of the aloe vera nanofibers were also changed significantly due to presence of the phages. Also, thermal stability and flexibility of the nanofibers were improved after containing the phage particles, because of higher thermodynamical and mechanical parameters of the phages in comparison to aloe vera substances. The antimicrobial properties of the aloe vera nanofibers which contained bacteriophages were significantly increased due to synergetic antimicrobial properties of the phages when electrospun with the aloe vera nanofibers. These findings suggested the nanofibers as hybrid nanomaterials for employing in food packaging.