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Tosco, Marisa; Orsenigo, Maria Novella; Esposito, Giovanni; Daelli, Alide
Biochimica et biophysica acta, 10/1988, Letnik: 944, Številka: 3Journal Article
Basolateral membrane vesicles from rat jejunal enterocytes, especially purified of brush-border contamination, were used for Na + uptake. The basolateral membrane vesicles are osmotically active and under our experimental conditions stimulates Na + uptake at both 5 μM and 5 mM concentrations. The proton gradient effect can be inhibited completely by 2 mM amiloride and partially by either FCCP or NH 4Cl (NH 3 diffusion). Membrane potential effects can be excluded by having valinomycin plus K + on both sides of the vesicles. These results suggest that there is an Na +/H + exchanger in the basolateral membrane of rat enterocytes.
