•Glossy blades from the Dalmatian Neolithic have been analysed.•Results demonstrate a change in harvesting techniques during the Middle Neolithic.•This change resulted in tools that would be quicker to resharpen.•This data raises the question about an increase in agricultural production. The Dalmatian coast has been occupied by Neolithic seafaring communities since the beginning of the 6th millennium BC. On the basis of pottery styles, a transition between the Impressed Ware and Danilo culture is defined, around 5300 cal BC. Nevertheless, bioarchaeological and archaeological data indicate general continuity between the two periods. In the current paper, we present an integrated technological and use-wear study of a large selection of lithics from several sites of both inland and insular Dalmatia. The results provide strong evidence for a change in harvesting techniques between the Impressed Ware and Danilo cultural phases. This change can be related to the pursuit of better performing inserts that could be used for longer periods and would be quicker to resharpen. Such a switch may have been associated with an initial increase in agricultural production during the Middle Neolithic.