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Wang, Xinwei; Zhao, Xiao
MedComm - Biomaterials and applications, June 2022, 2022-06-00, 20220601, 2022-06-01, Letnik: 1, Številka: 1Journal Article
As an important part of driving natural life systems, the function of protein networks is accurately controlled through many parameters, like distance, quantity, position, and orientation. Nevertheless, it would be very hard to control the physical arrangement of the multiple proteins to generate cellular signaling events or complex enzymatic cascades, for instance small molecule organic synthesis DNA nanotechnology provides matching nanoscale dimensions, the special programmability of DNA, and the capability and compatibility of many proteins and nucleic acids. DNA origami has precise addressing capabilities at the nanoscale, which ensures the accurate assembly of the protein networks. These characteristics indicate that the DNA origami is a highly addressable programmable nanomaterial, which can be applied for building artificial protein networks. Up to now, researchers have achieved significant progress in the establishment and application of the DNA origami‐protein networks. In the current review, we introduce the superiorities of DNA origami‐protein networks in detail, concluded their construction strategies, and their recent progression and applications in biomedicine and biophysics. In the end, we look into the future prospects of DNA origami‐protein networks. Finally, we looked forward to the future perspective of DNA origami‐protein networks. The staple strands (ssDNA strands) are able to self‐assembly with scaffold strands to create 2D, 3D DNA origami base. Programmatically conjugate of desired protein onto these DNA origami can achieve the goal of addressable precise assembly of protein networks.
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in: SICRIS
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