The current study aims to explore the antimicrobial and anticancer activity of biogenic silver nanoparticles (B-AgNPs) synthesized using Bael leaf extract. AgNPs nanoparticles characterized using FESEM, TEM, XRD, and UV-Spectrophotometry. TEM analysis revealed that B-AgNPs were predominantly spheroidal with particle size distribution in the range of 7-10 nm. In MTT assay, when B-AgNPs were applied in colon cancer (HCT-116) cells and human colon epithelial cell line (FHC) cells. B-AgNPs exhibited specific cytotoxicity to HCT-116 as minimal effect observed in normal FHC cells. Moreover, the fluorescence microscopic examination after nucleus staining as well as DNA fragmentation also confirmed AgNPs spectific cytotoxicity towards HCT-116. Further, AgNPs demonstrated excellent antibiofilm and antibacterial activity in both Gram-Positive and Gram-negative bacteria. Hence, overall, we categorically concluded that the biogenic silver nanoparticles synthesized using Bael leaf extract possess multiple biological activities and might be possibly used as multipurpose therapeutic material with no or little systemic toxicity.