Establishing an efficient and eco-friendly extraction method of bioactive ingredients from plant materials is highly needed to insure a better human health by combating different diseases. In this work, a two-phase system formed by green and recyclable menthol-based hydrophobic deep eutectic solvent (DES) aqueous was synthesized and evaluated its application for simultaneous extraction of seven main target taxanes in the Taxus chinese needles. The formation of micelles from two-phase system in the menthol-based DES aqueous simultaneously enhanced the mass transfer and enrichment for target taxanes. Under the optimal conditions of menthol/1-butanol ratio 1:1 (mol/mol), water content 50%, solid-liquid ratio 1:30 g/mL, extraction time 30 min and ultrasonic power 250 W, the extraction yields of seven main taxanes were 1.27–2.65 folds higher than the conventional organic solvents. The recovery yields after enrichment with the two-phase system of the menthol-based DES aqueous were higher than 95.31%. Furthermore, the menthol-based DES aqueous could be recycled at least three times with perfect extraction efficiency for target compounds. This work proved that the natural menthol-based DES aqueous solution is an effective and non-toxic green extraction solvent, which has the potential to effectively extract phytochemicals from plant materials in the future. Display omitted •Some menthol-based DES aqueous were prepared as green solvents.•Extraction and enrichment of taxanes could be conducted simultaneously.•The DES aqueous possessed higher reusability.•The kinetics of DES aqueous extraction process for taxanes was studied firstly.