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Biloivan, O.A.; Dzyadevych, S.V.; El'skaya, A.V.; Jaffrezic-Renault, N.; Zine, N.; Bausells, J.; Samitier, J.; Errachid, A.
Sensors and actuators. B, Chemical, 05/2007, Letnik: 123, Številka: 2Journal Article
A novel electrochemical microbiosensor for protein determination based on an all-solid-state microelectrodes with a Ppy 3,3-Co(1,2-C 2B 9H 11) 2 solid internal contact and immobilized trypsin and carboxypeptidase B was developed. The specificity of the sensor signal was investigated using artificial ester and amide substrates of the proteinases: Nα-benzoyl- l-arginine ethyl ester hydrochloride (BAEE), Nα-benzoyl- dl-arginine- p-nitroanilide hydrochloride (BAPNA), and Hippuryl-Arg. Study on a possibility of protein detection showed that quantitative proteins determination can be performed with the microbiosensor in the concentration range of 1–60 μg/ml.
