At a constant power output, cyclists prefer to use a higher cadence than those that minimize metabolic cost. The neuromuscular mechanism underpinning the preferred higher cadence remains unclear. PURPOSEThe aim of this study was to investigate the effect of cadence on joint level work and vastus lateralis (VL) fascicle mechanics while cycling at a constant, submaximal, power output. We hypothesized that preferred cycling cadence would enhance the power capacity of the VL muscle when compared with a more economical cadence. Furthermore, we predicted that the most economical cadence would coincide with minimal total electromyographic activity from the leg muscles. METHODSMetabolic cost, lower-limb kinematics, joint level work, VL fascicle mechanics, and muscle activation of the VL, rectus femoris, biceps femoris, gastrocnemius medialis, and soleus muscles were measured during cycling at a constant power output of 2.5 W·kg and cadences of 40, 60, 80, and 100 rpm. A preferred condition was also performed where cadence feedback was hidden from the participant. RESULTSMetabolic cost was lowest at 60 rpm, but the mean preferred cadence was 81 rpm. The distribution of joint work remained constant across cadences, with the majority of positive work being performed at the knee. The preferred cadence coincided with the highest VL power capacity, without a significant penalty to efficiency, based on fascicle shortening velocity. CONCLUSIONSCycling at a higher cadence is preferred to ensure that the muscle’s ability to produce positive power remains high. Further investigations are required to examine what feedback mechanism could be responsible for the optimization of this motor pattern.