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Sunshine, Joel C; Nguyen, Peter L; Kaunitz, Genevieve J; Cottrell, Tricia R; Berry, Sneha; Esandrio, Jessica; Xu, Haiying; Ogurtsova, Aleksandra; Bleich, Karen B; Cornish, Toby C; Lipson, Evan J; Anders, Robert A; Taube, Janis M
Clinical cancer research, 08/2017, Letnik: 23, Številka: 16Journal Article
PD-L1 expression in the pretreatment tumor microenvironment enriches for response to anti-PD-1/PD-L1 therapies. The purpose of this study was to quantitatively compare the performance of five monoclonal anti-PD-L1 antibodies used in recent landmark publications. PD-L1 IHC was performed on 34 formalin-fixed paraffin-embedded archival melanoma samples using the 5H1, SP142, 28-8, 22C3, and SP263 clones. The percentage of total cells (including melanocytes and immune cells) demonstrating cell surface PD-L1 staining, as well as intensity measurements/ -scores, were assessed for each melanoma specimen using a computer-assisted platform. Staining properties were compared between antibodies. Strong correlations were observed between the percentage of PD-L1(+) cells across all clones studied ( = 0.81-0.96). When present, discordant results were attributable to geographic heterogeneity of the melanoma tissue section rather than differences in PD-L1 antibody staining characteristics. PD-L1 intensity/ -scores strongly correlated with percentage of PD-L1(+) cells ( > 0.78, all clones). The 5H1, SP142, 28-8, 22C3, and SP263 clones all demonstrated similar performance characteristics when used in a standardized IHC assay on melanoma specimens. Reported differences in PD-L1 IHC assays using these antibodies are thus most likely due to assay characteristics beyond the antibody itself. Our findings also argue against the inclusion of an intensity/ -score in chromogenic PD-L1 IHC assays. .
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
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Vir: Osebne bibliografije
in: SICRIS
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