The wild species of rice, Oryza rufipogon , is a source of genetic variation for yield-enhancing traits and blast resistance. With the objective to genetically enhance the elite rice cultivar, ‘Samba Mahsuri’, a set of 192 BC 2 F 2 and BC 2 F 3 lines developed from O. rufipogon was characterized for yield-enhancing traits and blast resistance. The study revealed the presence of significant genetic variability and transgressive segregation for productive tillers, spikelets per panicle, and grain yield per plant. The study also identified several introgression lines superior to the recurrent parent for yield enhancing traits and blast resistance. Molecular characterization of 26 blast resistant lines identified multiple blast resistance genes in IL-136 Pib , Pi 9 (t), Pi54 , Pi 38, Pitp and Pi 20(t), IL-3 Pib , Pi54 , Pi 38, Pitp and Pi 20(t), and IL-140 Pib , Pi 9(t), Pi54 , Pi38 , and Pitp . Based on the performance of yield-enhancing traits and blast resistance, seven lines (IL-27, IL-28, IL-29, IL-72, IL-139, IL-158 and IL-171) showed merit. Estimation of the recovery of the recurrent parental genome by using 117 polymorphic SSR markers revealed that recurrent-parental genome recovery varied from 54.0 (IL-158) to 92.3% (IL-27). These promising, high-yielding introgression lines with multiple blast-resistance genes can serve as important genetic resources for yield and blast resistance improvement in rice, especially for the southern India.