Purpose This study aimed to develop a biocompatible oximetric electron paramagnetic resonance (EPR) spin probe with reduced self-relaxation, and sensitivity to oxygen for a higher signal-to-noise ratio and longer relaxation times at high oxygen concentration, compared to the reference spin probe OX071. Procedures SOX71 was synthesized by succinylation of the twelve alcohol groups of OX071 spin probe and characterized by EPR at X-Band (9.5 GHz) and at low field (720 MHz). The biocompatibility of SOX71 was tested in vitro and in vivo in mice. A pharmacokinetic study was performed to determine the best time frame for EPR imaging. Finally, a proof-of-concept EPR oxygen imaging was performed on a mouse model of a fibrosarcoma tumor. Results SOX71 was synthesized in one step from OX071. SOX71 exhibits a narrow line EPR spectrum with a peak-to-peak linewidth of 66 mG, similar to OX071. SOX71 does not bind to albumin nor show cell toxicity for the concentrations tested up to 5 mM. No toxicity was observed after systemic delivery via intraperitoneal injection in mice at twice the dose required for EPR imaging. After the injection, the probe is readily absorbed into the bloodstream, with a peak blood concentration half an hour, post-injection. Then, the probe is quickly cleared by the kidney with a half-life of ~ 45 min. SOX71 shows long relaxation times under anoxic condition (T 1e  = 9.5 µs and T 2e  = 5.1 µs; SOX71 = 1 mM in PBS at 37 °C, p O 2  = 0 mmHg, 720 MHz). Both the relaxation rates R 1e and R 2e show a decreased sensitivity to p O 2, leading to twice longer relaxation times under room air conditions ( p O 2  = 159 mmHg) compared to OX071. This is ideal for oxygen imaging in samples with a wide range of p O 2 . Both the relaxation rates R 1e and R 2e show a decreased sensitivity to self-relaxation compared to OX071, with a negligible effect of the probe concentration on R 1e . SOX71 was successfully applied to image oxygen in a tumor. Conclusion SOX71, a succinylated derivative of OX071 was synthesized, characterized, and applied for in vivo EPR tumor oxygen imaging. SOX71 is highly biocompatible, and shows decreased sensitivity to oxygen and self-relaxation. This first report suggests that SOX71 is superior to OX071 for absolute oxygen mapping under a broad range of p O 2 values.