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Perin, Emerson C., MD, PhD; Silva, Guilherme V., MD; Henry, Timothy D., MD; Cabreira-Hansen, Maria G., PhD; Moore, Warren H., MD; Coulter, Stephanie A., MD; Herlihy, J. Patrick, MD; Fernandes, Marlos R., MD; Cheong, Benjamin Y.C., MD; Flamm, Scott D., MD; Traverse, Jay H., MD; Zheng, Yi, MD; Smith, Deirdre, RN, CCRC; Shaw, Sandi, RN, BSN; Westbrook, Lynette, RN, MS, CCRC; Olson, Rachel, RN, MS, CCRC; Patel, Dipsu, MD; Gahremanpour, Amir, MD; Canales, John, MD; Vaughn, William K., PhD; Willerson, James T., MD
The American heart journal, 06/2011, Letnik: 161, Številka: 6Journal Article
Background Autologous bone marrow mononuclear cell (ABMMNC) therapy has shown promise in patients with heart failure (HF). Cell function analysis may be important in interpreting trial results. Methods In this prospective study, we evaluated the safety and efficacy of the transendocardial delivery of ABMMNCs in no-option patients with chronic HF. Efficacy was assessed by maximal myocardial oxygen consumption, single photon emission computed tomography, 2-dimensional echocardiography, and quality-of-life assessment (Minnesota Living with Heart Failure and Short Form 36). We also characterized patients' bone marrow cells by flow cytometry, colony-forming unit, and proliferative assays. Results Cell-treated (n = 20) and control patients (n = 10) were similar at baseline. The procedure was safe; adverse events were similar in both groups. Canadian Cardiovascular Society angina score improved significantly ( P = .001) in cell-treated patients, but function was not affected. Quality-of-life scores improved significantly at 6 months ( P = .009 Minnesota Living with Heart Failure and P = .002 physical component of Short Form 36) over baseline in cell-treated but not control patients. Single photon emission computed tomography data suggested a trend toward improved perfusion in cell-treated patients. The proportion of fixed defects significantly increased in control ( P = .02) but not in treated patients ( P = .16). Function of patients' bone marrow mononuclear cells was severely impaired. Stratifying cell results by age showed that younger patients (≤60 years) had significantly more mesenchymal progenitor cells (colony-forming unit fibroblasts) than patients >60 years (20.16 ± 14.6 vs 10.92 ± 7.8, P = .04). Furthermore, cell-treated younger patients had significantly improved maximal myocardial oxygen consumption (15 ± 5.8, 18.6 ± 2.7, and 17 ± 3.7 mL/kg per minute at baseline, 3 months, and 6 months, respectively) compared with similarly aged control patients (14.3 ± 2.5, 13.7 ± 3.7, and 14.6 ± 4.7 mL/kg per minute, P = .04). Conclusions ABMMNC therapy is safe and improves symptoms, quality of life, and possibly perfusion in patients with chronic HF.
