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Nagal, Vandana; Kumar, Virendra; Khan, Marya; AlOmar, Suliman Yousef; Tripathy, Nirmalya; Singh, Kedar; Khosla, Ajit; Ahmad, Naushad; Hafiz, Aurangzeb Khurram; Ahmad, Rafiq
New journal of chemistry, 10/2021, Letnik: 45, Številka: 4Journal Article
Uric acid (UA) level quantification is crucial for the diagnosis and treatment of cardiovascular, arthritis, renal disorder, and preeclampsia diseases. We report the solvent-assisted synthesis of zinc oxide (ZnO) nanoparticles (NPs) which we used to make a seed layer on a conductive fluorine-doped tin oxide (FTO) electrode. Vertically-arranged ZnO nanorods (NRs) were grown using a hydrothermal method. The ZnO NPs and NRs were characterized in detail, which revealed the smaller sizes (10-15 nm) of the NPs and the vertically-arranged nature of the NRs. Furthermore, a highly sensitive UA biosensor was constructed with vertically-arranged ZnO NRs. The electrochemical characterization of the UA biosensor (Nafion/uricase/ZnO NRs-ZnO NPs/FTO) using differential pulse voltammetry (DPV) demonstrated linearity over a wide UA concentration range (0.01-1.5 mM) with a high sensitivity (345.44 μA mM −1 cm −2 ) and limit of detection (LOD; 2.5 μM). The biosensor also showed good selectivity, high reproducibility, and enhanced shelf-life. Additionally, practical feasibility was tested in a real ( i.e. , human blood serum) sample. The boosted UA biosensor performance is attributed to the vertically-arranged ZnO NRs, which immobilize a large amount of enzyme owing to their high surface-to-volume ratio, compared to flat surface-based biosensors. The present strategy, using vertically-arranged ZnO NRs on NP-seeded electrodes, can be extended to fabricate different ZnO NR-based chemical/biosensing devices. Vertically-arranged ZnO nanorods grown on a ZnO nanoparticle-seeded FTO electrode using a hydrothermal method for highly sensitive uric acid biosensor fabrication.
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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in: SICRIS
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